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Merck

87748

3,3′,5,5′-Tetramethylbenzidine

TMB substrate, chromogenic, ≥98.0% (NT), powder

Synonym(s):

BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate

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About This Item

Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
54827-17-7
Molecular Weight:
240.34
NACRES:
NA.83
PubChem Substance ID:
329769606
UNSPSC Code:
12352204
EC Number:
259-364-6
MDL number:
MFCD00007748
Beilstein/REAXYS Number:
2808541

Key Documents

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Product Name

3,3′,5,5′-Tetramethylbenzidine, ≥98.0% (NT)

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

assay

≥98.0% (NT)

form

powder

technique(s)

UV/Vis spectroscopy: suitable

mp

166-171 °C, 168-171 °C (lit.)

storage temp.

2-8°C

Quality Level

300

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Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
3,3′,5,5′-Tetramethylbenzidine has been used as a substrate for peroxidase in:
  • enzyme-immunoassay (EIA)
  • indirect competitive enzyme-linked immunosorbent assay (ELISA)
  • sandwich enzyme-linked immunosorbent assay

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

87748-INTR: + 87748-5G: + 87748-1G: + 87748-VAR: + 2550035: + 87748-BULK: + 87748-25G:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH3716
BCCF7622
BCCK5557
BCCG6414
BCCD4813

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