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Merck

A3699

Acrylamide/Bis-acrylamide solution

30%, BioReagent, suitable for electrophoresis, 37.5:1

Synonym(s):

30% Acrylamide/Bisacrylamide 37.5:1, Polyacrylamide solution

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About This Item

UNSPSC Code:
41105319
PubChem Substance ID:
329770843
NACRES:
NA.25
MDL number:
MFCD00080848

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Product Name

Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 37.5:1

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

sterility

0.2 μm filtered

product line

BioReagent

form

liquid

feed ratio

37.5:1

technique(s)

electrophoresis: suitable

suitability

suitable for electrophoresis

storage temp.

2-8°C, 2-8°C

Quality Level

100

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General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide, 30% solution has been used:
  • in the preparation of polyacrylamide gel for photobleaching experiment
  • as a constituent in the preparation of gel precursor solution and pore-gradient microgel precursor solution
  • in the preparation of polyacrylamide gels during western blot analyses

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Deleterious substance

pdsc

Class I Designated Chemical Substances

prtr

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

A3699-100ML-PW: + A3699-5L:4548173314556 + A3699-BULK: + A3699-VAR: + A3699-PH: + A3699-5L-PW: + A3699PROC: + A3699-5X100ML:4548173185583 + A3699-100ML:4548173185576

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Certificates of Analysis (COA)

Lot/Batch Number
0000512673
0000512673
0000481784
0000481784
0000340944

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Single cell--resolution western blotting
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Current Biology, 27(16), 2420-2430 (2017)
Chi-Chih Kang et al.
Analytical chemistry, 86(20), 10429-10436 (2014-09-17)
Intratumor heterogeneity remains a major obstacle to effective cancer therapy and personalized medicine. Current understanding points to differential therapeutic response among subpopulations of tumor cells as a key challenge to successful treatment. To advance our understanding of how this heterogeneity
Pedram Madadkar et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1055-1056, 158-164 (2017-05-10)
Cation exchange (CEX) chromatography is widely used for large-scale separation of monoclonal antibody (mAb) aggregates. The aggregates bind more strongly to CEX media and hence elute after the monomeric mAb in a salt gradient. However, monomer-aggregate resolution that is typically
Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications
Ai HW, et al.
Nature Protocols, 9(4), 910-910 (2014)
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Related Content

Data Sheet - A3699

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