A7470
Anti-c-Myc Agarose Affinity Gel antibody produced in rabbit
affinity isolated antibody
Synonym(s):
Anti-c-Myc
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
agarose conjugate
Clone:
polyclonal
Application:
IP, affinity chromatography
Citations:
274
biological source
rabbit
conjugate
agarose conjugate
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
analyte chemical class(es)
proteins (c-Myc)
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
capacity
≥10 nmol/mL binding capacity (c-Myc tagged fusion protein), ≥7 nmol/mL elution capacity (c-Myc tagged fusion protein)
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
human ... MYC(4609)
General description
The human c-Myc proto-oncogene belongs to MYC oncogene family and is the human cellular homolog of the avian v-Myc gene found in several leukemogenic retroviruses. c-Myc gene is localized on human chromosome 8q24.21. The two major domains of include the N-terminal box regions and the C-terminal basic-region with helix-loop-helix/leucine-zipper motif.
Immunogen
synthetic peptide of the human p62c-Myc protein.
Application
Anti-c-Myc Agarose Affinity Gel antibody produced in rabbit has been used in:
- chromatin immunoprecipitation (ChIP)
- co-immunoprecipitation
- western blotting
- immunofluorescence
- immunoprecipitation
Biochem/physiol Actions
Anti-c-Myc recognizes an epitope located on c-Myc tagged fusion proteins.
c-Myc proto-oncogene increased expression is observed in a variety of human tumors. Amplification of Myc gene locus is observed in angiosarcomas and translocation is implicated in Burkitt′s lymphoma. c-Myc is a master regulator and targets a wide variety of metabolic events. An epitope located within amino acid residues 410-419 (EQKLISEEDL) of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tagged fusion proteins. Anti-c-Myc agarose is useful in the purification of expressed c-Myc tagged fusion proteins in bacterial lysates or transfected cells.
Physical form
Suspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
Preparation Note
For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
A7470-1ML-KC: + A7470-VAR: + A7470-1ML: + A7470-BULK:
jan
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Sachiko Takikawa et al.
Epigenetics, 8(12), 1268-1279 (2013-10-19)
Genomic imprinting is a common epigenetic phenomenon in mammals. Dysregulation of genomic imprinting has been implicated in a variety of human diseases. ZFP57 is a master regulator in genomic imprinting. Loss of ZFP57 causes loss of DNA methylation imprint at
Ritsuko Nakamura et al.
The Journal of biological chemistry, 287(5), 3282-3291 (2011-12-14)
Nel (neural epidermal growth factor (EGF)-like molecule) is a multimeric, multimodular extracellular glycoprotein with heparin-binding activity and structural similarities to thrombospondin-1. Nel is predominantly expressed in the nervous system and has been implicated in neuronal proliferation and differentiation, retinal axon
Robert C Alver et al.
Nucleic acids research, 42(13), 8389-8404 (2014-06-29)
Accurate replication of the genome requires the evolutionarily conserved minichromosome maintenance protein, Mcm10. Although the details of the precise role of Mcm10 in DNA replication are still debated, it interacts with the Mcm2-7 core helicase, the lagging strand polymerase, DNA
Riko Hatakeyama et al.
Molecular and cellular biology, 30(24), 5598-5607 (2010-10-20)
Endocytosis of nutrient transporters is stimulated under various conditions, such as elevated nutrient availability. In Saccharomyces cerevisiae, endocytosis is triggered by ubiquitination of transporters catalyzed by the E3 ubiquitin ligase Rsp5. However, how the ubiquitination is accelerated under certain conditions
Wei Wei et al.
Journal of virology, 88(1), 583-591 (2013-11-01)
Sterile alpha motif and HD domain-containing protein 1 (SAMHD1) restricts human immunodeficiency virus type 1 (HIV-1) infection in myeloid cells but is inactivated by certain classes of simian immunodeficiency virus (SIV) Vpx proteins. Vpx proteins recruit the DCAF1-CRL4 E3 ubiquitin
Related Content
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service