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Merck

B3408

6-Benzylaminopurine

suitable for plant cell culture

Synonym(s):

6-BAP, BA, N6-Benzyladenine

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About This Item

Empirical Formula (Hill Notation):
C12H11N5
CAS Number:
1214-39-7
Molecular Weight:
225.25
UNSPSC Code:
10171502
NACRES:
NA.72
PubChem Substance ID:
24891717
EC Number:
214-927-5
Beilstein/REAXYS Number:
19406
MDL number:
MFCD00005572
Form:
powder

Key Documents

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InChI key

NWBJYWHLCVSVIJ-UHFFFAOYSA-N

InChI

1S/C12H11N5/c1-2-4-9(5-3-1)6-13-11-10-12(15-7-14-10)17-8-16-11/h1-5,7-8H,6H2,(H2,13,14,15,16,17)

SMILES string

C(Nc1ncnc2nc[nH]c12)c3ccccc3

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

Quality Level

200

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General description

6-Benzylaminopurine is a plant growth regulator that belongs to the class of first generation synthetic cytokinin used in agriculture.

Application

6-Benzylaminopurine has been used:
  • to induce sprouting in plant materials
  • in seed germination medium for culturing of seeds
  • to modify Murashige and Skoog (MS) media for shoot initiation

6-Benzylaminopurine, benzyl adenine (BAP) is a synthetic cytokinin which together with auxins elicits plant growth and development responses. BAP is a widely use cytokinin supplement to plant growth media such as Murashige and Skoog medium, Gamborg′s medium, and Chu′s N6 medium.

Biochem/physiol Actions

BAP is an inhibitor of respiratory kinase in plants, and increases post-harvest life of green vegetables.

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Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Repr. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

B3408-VAR: + B3408-100MG: + B3408-BULK: + B3408-1G: + 2530032: + B3408-5G: + B3408-500MG: + B3408-25G:

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Certificates of Analysis (COA)

Lot/Batch Number
0000523146
0000523144
0000523144
0000523146
0000510867

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Callus induction and organogenesis in soybean [Glycine max (L.) Merr.] cv. Pyramid from mature cotyledons and embryos
Joyner E Y, et al.
The Open Plant Science Journal, 4(1) (2010)
In vitro propagation of `Guayabo del pais?(Acca sellowiana (Berg.) Burret)
Ross S and Grasso R
Fruit, Vegetable and Cereal Science and Biotechnology, 4(special issue 1), 83-87 (2010)
Establishment of analytical method for 6-benzylaminopurine residue, a plant growth regulator for brown rice, mandarin, pepper, potato, and soybean by using GC/NPD
Lee SM, et al.
Journal of the Korean Society for Applied Biological Chemistry, 57(1), 83-89 (2014)
In vitro plant regeneration from commercial cultivars of soybean
Raza G, et al.
BioMed Research International, 2017 (2017)
Fatima Naim et al.
PloS one, 15(1), e0227994-e0227994 (2020-01-25)
Introducing a new trait into a crop through conventional breeding commonly takes decades, but recently developed genome sequence modification technology has the potential to accelerate this process. One of these new breeding technologies relies on an RNA-directed DNA nuclease (CRISPR/Cas9)
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Protocols

Antibiotics in Plant Tissue Culture Protocol

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

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