B3640
Anti-Biotin antibody produced in goat
affinity isolated antibody, lyophilized powder
Synonym(s):
Anti-Biotin, Anti Biotin Antibody, Anti Biotin Antibody - Anti-Biotin antibody produced in goat, Anti-Biotin Antibody
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunoelectrophoresis
Technique(s):
immunoelectrophoresis: suitable
Citations:
33
Product Name
Anti-Biotin antibody produced in goat, affinity isolated antibody, lyophilized powder
biological source
goat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
technique(s)
immunoelectrophoresis: suitable
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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Application
Anti-Biotin antibody produced in goat has been used in multi-well plate assay, to identify desthiobiotinylating (analog of biotin) proteins. It has also been used in Colourimetric enzyme immunoassay.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
May be substituted for avidin to decrease non-specific binding.
Biochem/physiol Actions
Biotin serves as a cofactor for carboxylases, necessary for oxidative metabolism. Biotin absorption in small intestine is reduced on consumption of avidin rich food such as egg white. Thus, greatly decreasing its bioavailability, leading to egg white injury. Reduced levels of biotin is normally observed during pregnancy. Biotin deficiency might be congenital, with defective proteins involved in biotin homeostasis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Biotin is considered as an essential nutrient, though it is know to be secreted by some of the intestinal bacteria. It is found in liver and brain tissues in minute quantities.
Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.
Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.
Immunogen
Biotin-KLH conjugate
Physical form
Lyophilized from 0.01 M sodium phosphate, 15 mM sodium chloride, pH 7.2
Preparation Note
Reconstitute with 0.135 M sodium chloride.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
B3640-BULK: + B3640-BULK-V: + B3640-2MG: + B3640-1MG-PW: + B3640-VAR: + B3640PROC: + B3640-1MG: + B3640-PH: + B3640-5X1MG: + B3640-BULK-N: + B3640-VAR-PW:
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