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Merck

C5467

EX-CELL® ACF CHO Medium

Animal-component free, with HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

CHO Medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD01634638

Key Documents

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description

for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

quality

Drug/Device Master File available

technique(s)

cell culture | mammalian: suitable

components

HEPES: yes
NaHCO3: yes
phenol red: no
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

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Application

Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.

Features and Benefits

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity. Cell densities in excess of 8 × 106 cells/ml have been achieved in batch culture systems. Increases in protein productivity up to 500% per cell have been observed.

Preparation Note

Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use.

Other Notes

Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

C5467-5L: + C5467-200L: + C5467-1L: + C5467-100L: + C5467-20L: + C5467PROC: + C5467-50L: + C5467-500ML: + C5467-10L: + C5467-BULK: + C5467-1B5100: + C5467-200L5100: + C5467-6X1L: + C5467-PH:

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Certificates of Analysis (COA)

Lot/Batch Number
0000288080
0000288080
RNBL8517
RNBL3238
RNBK8042

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Articles

Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

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