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Merck

C7901

Chelex® 100 sodium form

50-100 mesh (dry)

Synonym(s):

Iminodiacetic Acid Resin

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About This Item

CAS Number:
UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
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form

beads

matrix

1% cross-linked polystyrene, cross-linked polystyrene beads

matrix active group

iminodiacetic acid (sodium form), paired iminodiacetate groups

matrix attachment

through methyl group to aromatic rings

particle size

50-100 mesh (dry)

pH

4-14

capacity

≥0.3 meq/mL binding capacity, 0.6 meq/g binding capacity (heavy metal ions)

Quality Level

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Application

Chelex is used in affinity chromatography, protein chromatography, chelating resins, affinity resins and core bioreagents.
Chelex® 100 sodium form has been used:
  • for the extraction of DNA from bee samples
  • to remove from cigarette smoke extract (CSE) and study the effects of hepcidin expression in the murine lungs
  • to extract DNA from bacterial cultures

Biochem/physiol Actions

Chelex® 100 is a styrene-divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions. It is considered a weakly acidic resin. Chelex acts as a chelating resin that is used in DNA extraction from forensic samples, such as hair, bloodstain cards, and buccal swabs.

Other Notes

Product is autoclavable in sodium form. It has a maximum operating temperature of 75°C and is not soluble in water.

Legal Information

Chelex is a registered trademark of Bio-Rad Laboratories, Inc.

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

C7901-25G: + C7901-BULK: + C7901-100G: + C7901-VAR: + C7901-50G: + C7901-500G:

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T P Ryan et al.
Journal of biochemical toxicology, 8(1), 33-39 (1993-03-01)
The effects of transition metals on nonenzymatic and ceruloplasmin catalyzed epinephrine oxidation were investigated by studying rates of epinephrine oxidation in purified buffers and in the presence of metal chelating agents. We found that epinephrine does not "autoxidize" in sodium
Methods for extracting genomic DNA from whole blood samples: current perspectives
Chacon Cortes D, et al.
Journal of Biosciences, 2014(2), 1-9 (2014)
Simultaneous detection of Escherichia coli O175: H7, Salmonella spp., and Listeria monocytogenes by multiplex PCR
Germini A, et al.
Food Control, 20(8), 733-738 (2009)
Elizabeth Perez et al.
Journal of immunology (Baltimore, Md. : 1950), 205(9), 2489-2498 (2020-09-23)
Chronic obstructive pulmonary disease (COPD) is a debilitating lung disease associated with cigarette smoking. Alterations in local lung and systemic iron regulation are associated with disease progression and pathogenesis. Hepcidin, an iron regulatory peptide hormone, is altered in subjects with
Vit Molva et al.
Insect science, 27(2), 266-275 (2018-08-14)
The interaction of house dust mites (HDM) and microorganisms is the key factor in the survival of these mites in human-made environments. Spent growth medium (SPGM) provides the rest of the diet, along with dead mite bodies and microorganisms. SPGM

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