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Merck

C8772

Cholera filtrate

lyophilized powder, bacterial sialidase receptor-destroying enzyme (RDE)

Synonym(s):

Cholera Filter

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
Form:
lyophilized powder
Quality level:
300

Key Documents

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Product Name

Cholera filtrate, lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

Quality Level

300

Related Categories

General description

Cholera filtrate promotes the activation of adenylate cyclase. It does not affect the activity of cyclic adenosine monophosphate (cAMP) phosphodiesterase.

Application

Cholerafiltrate has been used as a receptor destroying enzyme (RDE): inhemagglutination inhibition assay of serum samples, in microneutralizationassay of mice serum samples, in hemagglutination inhibition assay to removenon-specific inhibitors from the cell culture supernatant samples

Biochem/physiol Actions

Cholera filtrate is a bacterial sialidase receptor-destroying enzyme (RDE) and may be used as crude neuraminidase. It may also be used in serological testing for influenza.

Preparation Note

After reconstituting with 5ml sterile water, it will contain 0.01% MIT as preservative.

Other Notes

See a separate listing of neuraminidase for preparations showing higher activity.

Disclaimer

For research use only. Not for use in diagnostic procedures.

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Danger

flash_point_f

Not applicable

flash_point_c

Not applicable

supp_hazards

EUH071

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1A - Skin Sens. 1

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

C8772-1VL-PW: + C8772-BULK: + C8772-1VL: + C8772-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000490167
0000490167
0000403428
0000403428
0000149683

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WHO Tech Rep. Serv.
WHO Expert Committee on Influenza, 64 (1953)
Applying valency-based immuno-selection to generate broadly cross-reactive antibodies against influenza hemagglutinins
Daniela M, et al.
Nature Communications (2024)
A V Kornev et al.
Biulleten' eksperimental'noi biologii i meditsiny, 88(10), 414-416 (1979-10-01)
An in vitro model using homogenates of the rat intestine and liver for studying the V. Cholerae culture filtrate effect on the adenylate cyclase system is proposed. Optimal conditions for the adenylate cyclase functioning have been investigated for this model.
Maria Zimmermann et al.
Frontiers in immunology, 10, 829-829 (2019-05-02)
Determining antigen specificity is vital for understanding B cell biology and for producing human monoclonal antibodies. We describe here a powerful method for identifying B cells that recognize membrane antigens expressed on cells. The technique depends on two characteristics of
A Riccomi et al.
Vaccines, 12(4) (2024-04-27)
A number of studies have suggested that influenza vaccination can provide protection against COVID-19, but the underlying mechanisms that could explain this association are still unclear. In this study, the effect of the 2021/2022 seasonal influenza vaccination on the immune
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