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C9205

Anti-Glial Fibrillary Acidic Protein (GFAP)−Cy3™ antibody, Mouse monoclonal

clone G-A-5, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse, Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP)−Cy3™ antibody produced in mouse, Anti-ALXDRD, Anti-GFAP

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
CY3 conjugate
Clone:
G-A-5, monoclonal
Application:
IF (d)
Citations:
236
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biological source

mouse

Quality Level

200

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

G-A-5, monoclonal

form

buffered aqueous solution

species reactivity

human, pig, rat

technique(s)

direct immunofluorescence: 1:400 using alcohol-fixed, paraffin-embedded rat brain sections

isotype

IgG1

UniProt accession no.

P14136

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... GFAP(2670)
rat ... Gfap(24387)

General description

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 is a purified mouse monoclonal antibody conjugated to Cy3 reactive dye. It is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. GFAP, in the central nervous system, is found in developing, normal, reactive and neoplastic astrocytes and ependymal cells. It is expressed in developing oligodendrocytes in the peripheral nervous system. GFAP is found in some Schwann cells, enteric glial cells, Müller glia of the retina, and the satellite cells of human sensory ganglia. It has been demonstrated in some non-glial cells as well.

Immunogen

GFAP from pig spinal cord

Application

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 has been used in
  • immunohistochemistry
  • immunoblotting
  • immunolabelling,
  • immunocytochemistry
  • immunofluorescence

Biochem/physiol Actions

Glial Fibrillary Acidic Protein (GFAP) is expressed in several glial-related intracranial neoplasms such as astrocytomas and to a variable extent in ependymomas, medulloblastomas, choroid plexus papillomas, gangliogliomas, mixed gliomas, hemangioblastomas, pineocytomas, pineoblastomas, pituitary adenomas, retinoblastomas, oligodendrogliomas and in a small minority of peripheral nerve sheath tumors (schwannomas and neurofibromas). It may also be found in pleomorphic adenomas and ovarian and testicular teratomas.
The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, Bergmann glia cells and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.
The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

C9205-.5ML: + C9205-.2ML: + C9205-VAR: + C9205-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
0000541676
0000524177
0000524177
0000541676
0000415055

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