CAPHOS
Calcium Phosphate Transfection Kit
Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells
Synonym(s):
Gene delivery
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About This Item
grade
Molecular Biology
Quality Level
form
solution
usage
kit sufficient for 160 transfections (6 cm dishes), kit sufficient for 400 transfections (3.5 cm dishes), kit sufficient for 80 transfections (10 cm dishes)
technique(s)
transfection: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Application
- to enable transfection
- to transfect Hek293T cells
- to transfect H29D cells
BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D
Biochem/physiol Actions
Features and Benefits
- Suitable for transient and stable transfection
- Reproducible for a wide range of cell types
- Widely referenced
- Inexpensive
Other Notes
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 1
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Please refer to KIT Component information
pdsc
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prtr
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fsl
Please refer to KIT Component information
ishl_indicated
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ishl_notified
Please refer to KIT Component information
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Protocols
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
Articles
This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.
トランスフェクションは、遺伝子を細胞に導入して、遺伝子発現や細胞生物学の研究に役立ちます。
このウェビナーでは、トランスフェクションの概要および真核細胞にDNAやRNAを導入する方法について説明します。
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Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.