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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
form
powder
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high
shipped in
ambient
storage temp.
2-8°C
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Preparation Note
Formulated to contain 17.3 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate.
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Substances Subject to be Indicated Names
ishl_indicated
Substances Subject to be Notified Names
ishl_notified
D1152-BULK: + D1152-10X1L: + D1152-PROC: + D1152LF1-BIBC: + D1152-VAR: + D1152-BIBC: + D1152-25KG: + D1152-50L: + D1152-1L: + D1152-2X5L: + D1152-50K: + D1152-25K: + D1152-50KG: + D1152-10L:
jan
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Kiran Pandey et al.
Animal reproduction science, 181, 93-102 (2017-04-24)
Gonadotropin-releasing hormone receptors (GnRHRs) colocalize with insulin and glucocorticoid receptors in lipid rafts of the gonadotroph plasma membrane, where they facilitate downstream signaling. We recently found that orphan G-protein-coupled receptor (GPR)61 is expressed in the anterior pituitary (AP) of heifers
Importance of ROS-mediated autophagy in determining apoptotic cell death induced by physapubescin B.
Jian Xu et al.
Redox biology, 12, 198-207 (2017-03-05)
Physapubescin B, a steroidal compound extracted from the plant Physalis pubescens L. (Solanaceae), has been reported to possess anti-cancer potential, whereas the molecular mechanism remains elusive. In this study, we first demonstrated that physapubescin B induced autophagy in human cancer
Peiyi Yan et al.
The American journal of Chinese medicine, 46(3), 673-687 (2018-04-05)
Curcumin is a hydrophobic polyphenol derived from turmeric: the rhizome of the herb Curcumalonga. Autophagy is an evolutionarily conserved process, in which cellular proteins and organelles are engulfed in autophagosome and then fuses with lysosome for degradation. Our previous study