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Merck

D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

Synonym(s):

DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I

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About This Item

CAS Number:
9003-98-9
UNSPSC Code:
12352204
NACRES:
NA.53
EC Number:
232-667-0
MDL number:
MFCD00130918
EC Number:
3.1.21.1 (BRENDA, IUBMB)
Specific activity:
10000 units/mg protein





Biological source:
bovine pancreas
Concentration:
5—15 mg protein/mL

Key Documents

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biological source

bovine pancreas

grade

Molecular Biology

form

buffered aqueous glycerol solution

specific activity

10000 units/mg protein





mol wt

29.1 kDa

concentration

5—15 mg protein/mL

technique(s)

DNA extraction: suitable

suitability

suitable for molecular biology

UniProt accession no.

P00639

application(s)

cell analysis

foreign activity

RNase, none detected

storage temp.

−20°C

Quality Level

200

Gene Information

cow ... DNASE1(282217)

Application

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.
Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

General description

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.

Other Notes

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.
One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.
RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

D7291-.5MG: + D7291-BULK: + D7291-2MG: + D7291-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000507872
0000507872
0000454444
0000454444
SLCQ3894

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Umesh Kumar Dhawan et al.
Arteriosclerosis, thrombosis, and vascular biology, 41(10), 2598-2615 (2021-08-06)
Objective: Hypercholesterolemia-induced NETosis and accumulation of neutrophil extracellular traps (NETs) in the atherosclerotic lesion exacerbates inflammation and is causally implicated in plaque progression. We investigated whether hypercholesterolemia additionally impairs the clearance of NETs mediated by endonucleases such as DNase1 and
A Deoxyribonuclease from Chlamydomonas reinhardii:Purification and Properties
TAIT GCL and HARRIS WJ
European Journal of Biochemistry, 75(2), 357-364 (1977)
Stage-Specific Expression Profiles of Anti-Mullerian Hormone and its Type II Receptor in Germ Cells during Spermatogenic Cycle of Rats
Ohyama K, et al.
Journal of Steroids & Hormonal Science, 8(187), 2-2 (2017)
Expression of anti-Mullerian hormone and its type II receptor in germ cells of maturing rat testis
Ohyama K, et al.
Endocrine Journal, EJ15-0370 (2015)
S Homburg et al.
The Journal of cell biology, 150(2), 293-307 (2000-07-26)
We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein
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