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Merck

D9154

o-Dianisidine dihydrochloride

tablet

Synonym(s):

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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About This Item

Empirical Formula (Hill Notation):
C14H16N2O2 · 2HCl
CAS Number:
20325-40-0
Molecular Weight:
317.21
NACRES:
NA.83
PubChem Substance ID:
24894263
UNSPSC Code:
12352204
EC Number:
243-737-5
MDL number:
MFCD00012488
Beilstein/REAXYS Number:
3917996

Key Documents

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Product Name

o-Dianisidine dihydrochloride, tablet, 10 mg substrate per tablet

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI key

UXTIAFYTYOEQHV-UHFFFAOYSA-N

SMILES string

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

form

tablet

solubility

water: 1 tablet/10 mL, clear, colorless

storage temp.

2-8°C

Quality Level

200

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Biochem/physiol Actions

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Preparation Note

Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.

Disclaimer

Probably carcinogenic.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

D9154-BULK: + D9154-50TAB:4548173224251 + D9154-100TAB:4548173224244 + D9154-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000390713
0000390713
SLCJ7840
SLCD6994
SLBX3894

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M Kaszuba et al.
Biochimica et biophysica acta, 1419(2), 221-228 (1999-07-17)
Reactive cationic and anionic liposomes have been prepared from mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol incorporating dimethyldioctadecylammonium bromide and DMPC incorporating phosphatidylinositol, respectively. The liposomes were prepared by the vesicle extrusion technique and had the enzymes glucose oxidase (GO) encapsulated
Frédéric Frottin et al.
Molecular & cellular proteomics : MCP, 5(12), 2336-2349 (2006-09-12)
Methionine aminopeptidase (MAP) is a ubiquitous, essential enzyme involved in protein N-terminal methionine excision. According to the generally accepted cleavage rules for MAP, this enzyme cleaves all proteins with small side chains on the residue in the second position (P1')
Cathrine Nordgaard et al.
International journal of molecular sciences, 22(17) (2021-09-11)
p38 and c-Jun N-terninal kinase (JNK) are activated in response to acute stress and inflammatory signals. Through modification of a plethora of substrates, these kinases profoundly re-shape cellular physiology for the optimal response to a harmful environment and/or an inflammatory
Rémy Ricoux et al.
Bioconjugate chemistry, 19(4), 899-910 (2008-03-08)
To develop artificial hemoproteins that could lead to new selective oxidation biocatalysts, a strategy based on the insertion of various iron-porphyrin cofactors into Xylanase A (Xln10A) was chosen. This protein has a globally positive charge and a wide enough active
Lori Hartnett et al.
The International journal of developmental biology, 54(4), 573-583 (2009-09-17)
The insulin-like growth factor (IGF) family is essential for normal embryonic growth and development and it is highly conserved through vertebrate evolution. However, the roles that the individual members of the IGF family play in embryonic development have not been
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