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Merck

F2878

Ficoll® PM 70

Type 70

Synonym(s):

Poly(sucrose-co-epichlorhydrin)

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About This Item

CAS Number:
72146-89-5
UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:
MFCD00131071

Key Documents

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type

Type 70

form

powder

mol wt

~70,000

color

white

solubility

H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

Quality Level

200

Related Categories

General description

Ficoll® PM70, a high molecular weight branched polysaccharide polymer, is believed that they do not contain any ionized groups. This sucrose-polymer is formed by the copolymerization of sucrose with epichlorhydrin.

Application

Ficoll® PM 70 has been used:
  • as a macromolecule in a comparative study to evaluate its effect in vitrification solutions
  • as a supplement in the vitrification solution for the vitrification procedure of oocytes
  • as a component of low serum medium for human lung fibroblast cell culture to promote a crowded environment

Biochem/physiol Actions

Ficoll is commonly used as a macromolecular constituent of vitrification solutions. Due to their dense branching, Ficolls also serve as ideal, nearly spherical molecular crowding agents.
FicollTM PM 70 is used as a macromolecular crowding agent in studies of cell volume signaling and protein refolding.It may be used in tissue engineering and macromolecular conformation research for the development, evaluation, and use of macromolecular crowding (MMC) systems and configurations. Ficoll PM-70 can also be used in vitrification research.

Other Notes

A nonionic synthetic polymer of sucrose.
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

F2878-50G: + F2878-500G: + F2878-100G: + F2878-5G: + F2878-250G: + F2878-VAR: + F2878-BULK: + F2878-25G:

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Certificates of Analysis (COA)

Lot/Batch Number
0000533685
0000533684
0000533685
0000533684
0000503188

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Emmanuel Dauty et al.
Journal of molecular recognition : JMR, 17(5), 441-447 (2004-09-14)
Aqueous environments in living cells are crowded, with up to >50 wt% small and macromolecule-size solutes. We investigated quantitatively one important consequence of molecular crowding--reduced diffusion of biologically important solutes. Fluorescence correlation spectroscopy (FCS) was used to measure the diffusion
M B Burg
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 10(5-6), 251-256 (2000-12-23)
The non-ideal properties of solutions containing high concentrations of macromolecules can result in enormous increases in the activity of the individual macromolecules. There is considerable evidence that macromolecular crowding and confinement not only occur in cells, but that these are
M Al-Habori
The international journal of biochemistry & cell biology, 33(9), 844-864 (2001-07-20)
Macromolecular crowding has been proposed as a mechanism by means of which a cell can sense relatively small changes in volume or, more accurately, the concentration of intracellular solutes. According to the macromolecular theory, the kinetics and equilibria of enzymes
Nobuhiko Tokuriki et al.
Protein science : a publication of the Protein Society, 13(1), 125-133 (2003-12-24)
Unfolded states of ribonuclease A were used to investigate the effects of macromolecular crowding on macromolecular compactness and protein folding. The extent of protein folding and compactness were measured by circular dichroism spectroscopy, fluorescence correlation spectroscopy, and NMR spectroscopy in
Y Georgalis et al.
Journal of colloid and interface science, 386(1), 141-147 (2012-08-23)
Ficoll PM70 is an important branched polysaccharide polymer with a variety of applications in biology and biophysics. The accepted picture for the aqueous solutions of this polymer is that of a polydisperse nearly spherical suspension with a molecular weight of
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Protocols

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

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