F4771
Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse
clone 1B10, ascites fluid
Synonym(s):
Fibroblast Surface Protein Antibody, Fibroblast Surface Protein Antibody - Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse
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About This Item
Conjugate:
unconjugated
Clone:
1B10, monoclonal
Application:
FACS, IHC (f), complement-mediated cytotoxicity assay
Citations:
25
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1B10, monoclonal
mol wt
antigen 43-72-80 kDa
contains
15 mM sodium azide
species reactivity
human
technique(s)
complement-mediated cytotoxicity assay: 1:500, flow cytometry: suitable, immunohistochemistry (frozen sections): suitable
isotype
IgM
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
Monoclonal Anti-Human Fibroblast Surface Protein (mouse IgM isotype) is derived from the 1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cultured human thymic fibroblasts. Human fibroblast surface protein is a fibroblast antigen that is expressed mainly on human synovial, foreskin and thymic fibroblasts and is absent on human epithelial cells and lymphocytes.
This fibroblast antigen has been demonstrated on human synovial, mammary, foreskin and thymic fibroblasts and in malignant fibrosarcoma tissue. The antigen is both cell membrane and lysosome associated.
Immunogen
cultured human thymic fibroblasts
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse has been used in:
- immunofluorescence
- flow cytometry
- immunohistochemistry
- immunoblotting
Monoclonal anti-fibroblast surface protein antibody can be used in flow cytometry of human fibroblasts and monocytes. It is also useful in immunoblotting and western blotting.
Biochem/physiol Actions
Monoclonal Anti-Fibroblast Surface Protein antibody is also used to remove fibroblasts from human cultured cells by inhibiting fibroblasts adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells.
Analysis Note
Working dilution is at least 1:500 by complement-mediated microcytotoxicity assay.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
F4771-VAR: + F4771-BULK: + F4771-.5ML: + F4771-.2ML:
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M Olovsson et al.
Gynecologic and obstetric investigation, 49(3), 165-169 (2000-03-24)
To investigate the ability of human uterine myocytes to grow under anaerobic conditions for a prolonged time period. Cells were isolated from fundal myometrium and cultured until subconfluency. The cell type was confirmed by immunostaining for the smooth muscle cell-specific
Yuki Sekino et al.
FEBS open bio (2021-06-12)
There are no human cancer cell lines of external auditory canal origin available for research use. This report describes the establishment of a culture condition for external auditory canal squamous cell carcinoma, derived from human tumor tissue. Successive squamous cell
Qiyuan Zhou et al.
American journal of respiratory cell and molecular biology, 54(5), 728-739 (2015-10-22)
We have reported that von Hippel-Lindau protein (pVHL) expression is elevated in human and mouse fibrotic lungs and that overexpression of pVHL stimulates fibroblast proliferation. We sought to determine whether loss of pVHL in fibroblasts prevents injury and fibrosis in
Carcinoma-Associated Fibroblast-Like Differentiation of Human Mesenchymal Stem Cells
Mishra PJ, et al.
Cancer research, 68(11), 4331-4339 (2008)
Christian Hagel et al.
Neuropathology : official journal of the Japanese Society of Neuropathology, 32(4), 406-414 (2011-12-02)
The tumor suppressor disorder neurofibromatosis type 1 (NF1) is associated with development of multiple neurofibromas which may grow intraneurally as plexiform neurofibromas (PNF) or intracutaneously (CNF). Upon surgery neurofibromas may show prominent swelling hindering skin-edge approximation. To assess whether the
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