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Merck

F4890

Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse

clone RG-16, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)

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About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
RG-16, monoclonal
Application:
IF, IHC (p)
Citations:
17

Key Documents

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biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RG-16, monoclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

should not react with

pig, chicken, bovine, horse, guinea pig, turkey, rat, canine, goat, feline, sheep

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General description

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Application

Monoclonal Anti-Rabbit Immunoglobulins-FITC antibody produced in mouse has been used in:
  • immunocytochemistry
  • immunohistochemistry
  • immunofluorescence

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

Biochem/physiol Actions

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Physical form

Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

F4890-.2ML: + F4890-BULK: + F4890-.5ML: + F4890-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000417623
0000417623
0000324929
0000324929
0000249551

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Increased synthesis of heparin affin regulatory peptide in the perforant path lesioned mouse hippocampal formation
Poulsen FR, et al.
Experimental Brain Research. Experimentelle Hirnforschung. Experimentation Cerebrale, 135(3), 319-330 (2000)
A study of the process of apoptosis in animals infected with the contagious ecthyma virus
Garrido-Farina GI, et al.
Veterinary Microbiology, 129(1-2), 28-39 (2008)
Kunio Hieshima et al.
Journal of immunology (Baltimore, Md. : 1950), 180(2), 931-939 (2008-01-08)
Adult T cell leukemia is a mature CD4+ T cell malignancy which predominantly expresses CCR4 and is etiologically associated with human T cell leukemia virus type 1 (HTLV-1). Because HTLV-1 transmission depends on close cell-cell contacts, HTLV-1-infected T cells may
Gracjana Krzysiek-Maczka et al.
American journal of cancer research, 12(3), 1337-1371 (2022-04-13)
Despite of the improvement in gastric cancer (GC) therapies patients still suffer from cancer recurrence and metastasis. Recently, the high ratio of these events combined with increased chemoresistance has been related to the asymptomatic Helicobacter pylori (Hp) infections. The limited
S Gamen et al.
FEBS letters, 376(1-2), 15-18 (1995-11-27)
It has been proposed that TNF cytotoxicity is mediated by reactive oxygen intermediates generated by uncoupling of mitochondrial respiration. We have compared sensitive U937 cells and derived cell lines depleted of mtDNA for their ability to undergo TNF- and Fas-induced
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Related Content

Data Sheet - F4890

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