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Merck

F6755

Fibrinogen from rat plasma

60-80% protein (≥60% of protein is clottable)

Synonym(s):

Factor I

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About This Item

CAS Number:
9001-32-5
UNSPSC Code:
12352202
EC Number:
232-598-6
NACRES:
NA.61
MDL number:
MFCD00131060
Form:
powder
Biological source:
rat plasma
Mol wt:
α-chain 63.5 kDa, β-chain 56 kDa, γ chain 47 kDa (about 4% carbohydrate content), soluble dimer 340 kDa

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biological source

rat plasma

form

powder

quality

60-80% protein (≥60% of protein is clottable)

mol wt

α-chain 63.5 kDa, β-chain 56 kDa, γ chain 47 kDa (about 4% carbohydrate content), soluble dimer 340 kDa

concentration

60-80% (biuret)

technique(s)

cell culture | mammalian: suitable, immunoblotting: suitable

UniProt accession no.

P02680, P06399, P14480

storage temp.

−20°C

Quality Level

200

Gene Information

rat ... Fga(361969), Fgb(24366), Fgg(24367)

Application

Fibrinogen from rat plasma has been used:
  • in the preparation of growth factor solutions
  • to apply on discontinuous SDS-polyacrylamide gels for the detection of IgG (immunoglobulins) type antibodies by immunoblot analysis
  • to mix with cell suspension to form a clot and retain the cells for avoiding its dispersion during transplantation

Fibrinogen has been used in studies of haemostatic therapy in surgical and massive trauma patients. These studies have shown that fibrinogen may prove to be more superior in stopping blood loss when compared to using fresh frozen plasma (FFP).

Biochem/physiol Actions

Acute phase protein that is part of the coagulation cascade of proteins.
Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyses fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source.

Analysis Note

Contains ~12% sodium citrate and ~18% sodium chloride.
Protein determined by biuret.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

F6755-BULK: + F6755-BULK-N: + F6755-PH: + F6755PROC: + F6755-VAR: + F6755-5MG: + F6755-1MG: + F6755-25MG-PW: + F6755-100MG-PW: + F6755-100MG: + F6755-25MG:

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Certificates of Analysis (COA)

Lot/Batch Number
0000447593
0000447593
0000350758
0000350758
SLCF3879

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Cell Transplantation as a Method to Investigate Spinal Cord Regeneration in Regenerative and Nonregenerative Xenopus Stages
Mendez-Olivos EE and LarraIn J
Cold Spring Harbor Protocols (2018)
Optimization of trophic support for neural stem cell grafts in sites of spinal cord injury
Robinson J and Lu P
Experimental Neurology, 291, 87-97 (2017)
Salmon fibrin glue in rats: antibody studies
Laidmae I, et al.
Biologicals : Journal of the International Association of Biological Standardization, 40(1), 55-60 (2012)
C Fenger-Eriksen et al.
British journal of anaesthesia, 101(6), 769-773 (2008-09-27)
Patients experiencing massive haemorrhage are at high risk of developing coagulopathy through loss, consumption, and dilution of coagulation factors and platelets. It has been reported that plasma fibrinogen concentrations may reach a critical low level relatively early during bleeding, calling
Anna Agren et al.
Anesthesia and analgesia, 118(5), 933-935 (2014-05-02)
Fibrinogen is of crucial importance in patients with ongoing bleeding. In this study, we compared fibrinogen concentration measured by thrombelastography (TEG®) with fibrinogen plasma concentration determined by Clauss. Sixty-three surgical patients and 38 healthy controls were included. For the whole
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