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Merck

F7252

Fast Green FCF

Dye content ≥85 %, powder

Synonym(s):

Food green 3

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About This Item

Linear Formula:
C37H34N2O10S3Na2
CAS Number:
2353-45-9
Molecular Weight:
808.85
UNSPSC Code:
12352204
NACRES:
NA.83
PubChem Substance ID:
57654190
EC Number:
219-091-5
MDL number:
MFCD00013053
Colour Index Number:
42053
Beilstein/REAXYS Number:
5718212

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Product Name

Fast Green FCF, Dye content ≥85 %

InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O

InChI key

RZSYLLSAWYUBPE-UHFFFAOYSA-L

form

powder

composition

Dye content, ≥85%

mp

290 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL, clear

Quality Level

200

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General description

Fast Green FCF is one of the triarylmethane dyes, that is biocompatible. This triphenylmethane color additive is mainly used in coloring food, drugs and cosmetics.

Application

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Fast Green FCF has been used for in staining of menisci from rabbit and of osteochondral tissues.

Biochem/physiol Actions

Fast Green FCF can be considered as a safe stain to detect tumor. In recent years, this has gained importance as an important drug to treat amyloid-deposit diseases.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

hcodes

H335,H341

Hazard Classifications

Muta. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

352.4 °F - closed cup

flash_point_c

178 °C - closed cup

ppe

Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

F7252-25G: + F7252-BULK: + F7252-10G: + F7252-100G: + F7252-5G: + F7252-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS7862
SHBR0868
SHBP7363
SHBN0187
SHBM1390

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Sarah Zerimech et al.
Neuropharmacology, 166, 107951-107951 (2020-01-17)
Cortical spreading depression (CSD) is a wave of transient network hyperexcitability leading to long lasting depolarization and block of firing, which initiates focally and slowly propagates in the cerebral cortex. It causes migraine aura and it has been implicated in
Synthetic Food Colors
Corradini MG
Encyclopedia of Agriculture and Food Systems, 291-296 (2019)
Fast green FCF alleviates pain hypersensitivity and down-regulates the levels of spinal P2X4 expression and pro-inflammatory cytokines in a rodent inflammatory pain model
Xu F, et al.
Frontiers in Pharmacology, 9, 534-534 (2018)
Chitosan-glycerol phosphate/blood implants increase cell recruitment, transient vascularization and subchondral bone remodeling in drilled cartilage defects
Chevrier A, et al.
Osteoarthritis and Cartilage, 15(3), 316-327 (2007)
Eun Jung Lee et al.
Cell reports, 28(3), 712-722 (2019-07-18)
The homeodomain is found in hundreds of transcription factors that play roles in fate determination via cell-autonomous regulation of gene expression. However, some homeodomain-containing proteins (HPs) are thought to be secreted and penetrate neighboring cells to affect the recipient cell
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タンパク質染色試薬選択ガイド

Sigmaでは、タンパク質可視化のためのEZBlue™ 試薬とProteoSilver™ 試薬をご提供しています。プロテオミクスや従来のPAGEフォーマットに適しています。

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

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