Skip to Content
Merck

G100120

Sephadex® G-100

Medium

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

CAS Number:
9050-94-6
UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
MFCD00002744

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

technique(s)

LPLC: suitable

matrix

Agarose

matrix active group

polymer

swelling

1 g swells to 15-20 mL gel

bead size

40-120 μm

separation technique

size exclusion (SEC)

Quality Level

100

form

powder

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Sephadex® is composed of repeating glucose units attached by α-1,6 glucosidic bonds. This structure ensures more aptamer binding sites and greater binding capacity. It is suitable for large-scale purification and is considered stable and can be utilized more than once.
Fractionation Range (MW)
Globular Proteins: 4,000 - 150,000
Dextrans: 1,000 - 100,000

Application

Sephadex®G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography
It has been used:
  • in gel filtration column for α-amylase purification
  • in gel filtration column for the purification of polysaccharidic fraction extracted from the skin of the ray Raja radula
  • to remove excess protamine by size exclusion from cross-linked iron oxide (CLIO)-protamine-linked (B) peptides

Other Notes

G100120-100G′s updated product number is GE17-0060-01

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

G100120-50G: + G100120-100G: + G100120-BULK: + G100120-10G: + G100120-500G: + G100120-VAR:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000541932
0000541933
0000541933
0000541932
0000486237

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ahmed K A El-Sayed et al.
International journal of biological macromolecules, 132, 1274-1281 (2019-04-07)
AmyLa α-amylase gene from Laceyella sp. DS3 was heterologously expressed in E. coli BL21. E. coli BL21 maximally expressed AmyLa after 4 h of adding 0.02 mM IPTG at 37 °C. The recombinant AmyLa α-amylase was purified 2.19-fold through gel filtration and ion
Labeling of immune cells for in vivo imaging using magnetofluorescent nanoparticles
Pittet MJ, et al.
Nature Protocols, 1(1), 73-73 (2006)
Mohamed Ben Mansour et al.
Thrombosis research, 123(4), 671-678 (2008-07-12)
The polysaccharide fraction from the skin of the ray Raja radula was extracted, characterized and assayed for anticoagulant activity. A whole polysaccharidic fraction was extracted from the skin of the ray Raja radula by papain digestion followed by cetylpyridinium chloride
Molecular Basis of the Difference in Oxygen Affinity Between Maternal and Foetal Red Blood Cells in the Viviparous Garter Snake <I>Thanmophis Elegans</I>
Berner, N.J., and Ingermann, R.L.
The Journal of Experimental Biology, 140, 147-153 (1988)
Gene sequence, modeling, and enzymatic characterization of alpha-amylase AmyLa from the thermophile Laceyella sp. DS3
El-Sayed AKA, et al.
Starch, 69(5-6), 1600255-1600255 (2017)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service