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Merck

G25150

Sephadex® G-25

Medium

Synonym(s):

Sephadex G-25 Medium, Sephadex® G-25 resins, Size exclusion resins

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About This Item

CAS Number:
9041-35-4
UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
MFCD00132770

Key Documents

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SMILES string

O1[C@H]([C@@H]([C@@H]([C@@H]([C@H]1CO)O)O)O)O.OC(CO)CO

InChI

1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1

InChI key

BCPVZFFJGCURNR-OCOFDJSDSA-N

technique(s)

buffer exchange: suitable

matrix active group

phase

swelling

1 g swells to 4-6 mL

bead size

50-150 μm

application(s)

life science and biopharma

compatibility

Cytiva

Quality Level

200

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General description

Sephadex® G-25 Medium is a size exclusion chromatography or gel filtration resin that is created by crosslinking dextran with epichlorohydrin. The various types of Sephadex can differ in their degree of cross-linking, resulting in variances in their swelling capacity and molecular fractionation range. Sephadex G-25 is one option available among the different G-types of Sephadex. Sephadex G-25 belongs to a range of five G-types, catering to different molecule sizes. Specifically, G-10 is suitable for small molecules, while G-75 is designed to accommodate larger molecules. Sephadex G-25 provides industrial requirements by ensuring a dependable supply and offering extensive technical and regulatory assistance.

Application

Fractionation Range (MW)
Dextrans: 100 - 5,000
Globular Proteins 1,000 - 5,000

Sephadex® G-25 has been used:
  • in the desalting of protease
  • in the column for the separation of radioiodinated oTP-1 from free iodine during the characterization of radioiodinated oTP-1 and receptor assay validation
Sephadex® G-25 has been used to separate the labelled proteins from unreacted dye N-hydroxysuccinimide (NHS) esters by gel permeation chromatography.

Biochem/physiol Actions

Sephadex® G-25 is a well-established gel filtration resin, widely used for desalting and buffer exchange in industrial applications. Its hydrophilic matrix minimizes nonspecific adsorption, resulting in high recoveries during these processes for proteins and nucleic acids. Sephadex® G-25 is usually used in affinity chromatography, protein chromatography and gel filtration chromatography.

Features and Benefits

  • Rapid desalting, contaminant removal, and transfer to a new buffer in a single step.
  • High recovery rate with minimal sample dilution.
  • Offered in prepacked HiPrep Desalting and HiTrap desalting columns for convenient and fast desalting.
  • Specifically designed as a BioProcess resin for industrial applications.

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

G25150-500G-C: + G25150-CH: + G25150-500G: + G25150-BULK: + G25150-10G: + G25150-50G: + G25150-100G-C: + G25150-VAR: + G25150-100G:

jan

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Certificates of Analysis (COA)

Lot/Batch Number
0000507039
0000507038
0000507038
0000507039
0000486774

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Lixue Shi et al.
Nature biotechnology (2021-10-06)
Mapping the localization of multiple proteins in their native three-dimensional (3D) context would be useful across many areas of biomedicine, but multiplexed fluorescence imaging has limited intrinsic multiplexing capability, and most methods for increasing multiplexity can only be applied to
Monika A Szczygiel et al.
Biology of reproduction, 67(1), 287-292 (2002-06-25)
Success with in vitro fertilization (IVF) using inbred strains of mice varies considerably and appears to be related to the proportion of motile spermatozoa present in epididymal sperm samples of different strains. In this study, motile spermatozoa were separated from
Characterization of endometrial receptors for ovine trophoblast protein-1 during the estrous cycle and early pregnancy in sheep
Knickerbocker JJ and Niswender GD
Biology of Reproduction, 40(2), 361-369 (1989)
L R Hale et al.
Genetics, 129(1), 103-117 (1991-09-01)
Preliminary studies with restriction fragment length polymorphisms of mitochondrial DNA (mtDNA) in natural populations of Drosophila melanogaster revealed considerable variation in terms of nucleotide sequence and overall size. In this report we present data from more isofemale lines and more
K M Yocom et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(22), 7052-7055 (1982-11-01)
A stable complex between pentaammineruthenium(III) and histidine-33 in horse heart ferricytochrome c is formed in the reaction between aquopentaammineruthenium(II) and the protein at pH 7. HPLC of the tryptic hydrolysate of the modified protein was employed to identify the pentaammineruthenium
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