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Merck

G5160

β-Galactosidase from Aspergillus oryzae

≥8.0 units/mg solid

Synonym(s):

β-D-Galactoside galactohydrolase, Lactase from Aspergillus oryzae

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About This Item

CAS Number:
9031-11-2
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
MFCD22628840
EC Number:
3.2.1.23 (BRENDA, IUBMB)
Specific activity:
≥8.0 units/mg solid

Key Documents

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form

solid

specific activity

≥8.0 units/mg solid

contains

dextrin as extender

storage temp.

−20°C

Quality Level

200

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General description

β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.

Application

β-Galactosidase from Aspergillus oryzae has been used:
  • in in vitro nuclear magnet resonance (NMR)
  • as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase
  • to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties
  • for enzymatic hydrolysis of lactose
  • for reversed-phase (RP) adsorption
  • in the hydrolysis of whey lactose

Biochem/physiol Actions

β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Other Notes

One unit will hydrolyze 1.0 μmole of lactose per minute at pH at 4.5 at 30 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

G5160-25KU: + G5160-BULK: + G5160-VAR: + G5160-125KU: + G5160-500KU:

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Certificates of Analysis (COA)

Lot/Batch Number
0000511548
0000511550
0000511549
0000511550
0000511548

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Hydrolysis of whey lactose by immobilized β-Galactosidase
Marcela Panaro Mariotti; Hideko Yamanaka; Angela Regina Araujo; Henrique Celso Trevisan
Brazilian Archives of Biology and Technology, 51 (2008)
Modular process for the flexible synthesis of magnetic beads-Process and product validation
Birgit Hickstein1, and Urs Alexander Peuker
Journal of Applied Polymer Science, 112, 2366-2373 (2009)
Jian-Xin Yu et al.
NMR in biomedicine, 21(7), 704-712 (2008-02-22)
Gene therapy has emerged as a promising strategy for treatment of various diseases. However, widespread implementation is hampered by difficulties in assessing the success of transfection in the target tissue and the longevity of gene expression. Thus, there is increasing
Martin Dragosits et al.
Applied microbiology and biotechnology, 98(8), 3553-3567 (2013-09-17)
Galactosidases are widespread enzymes that are used for manifold applications, including production of prebiotics, biosynthesis of different transgalactosylated products, improving lactose tolerance and in various analytical approaches. The nature of these applications often require galactosidases to be present in a
C M Seubert et al.
Cancer gene therapy, 18(1), 42-52 (2010-09-11)
Breast cancer is the most common cause of cancer-related death worldwide, thus remaining a crucial health problem among women despite advances in conventional therapy. Therefore, new alternative strategies are needed for effective diagnosis and treatment. One approach is the use
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