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Merck

H3506

Hyaluronidase from bovine testes

Type I-S, lyophilized powder, 400-1000 units/mg solid

Synonym(s):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

CAS Number:
37326-33-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
MFCD00131351
EC Number:
3.2.1.35 (BRENDA, IUBMB)
Specific activity:
400-1000 units/mg solid

Key Documents

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type

Type I-S

form

lyophilized powder

specific activity

400-1000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

UniProt accession no.

Q5E985, Q8SQG8

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

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Application

Hyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.

Suitable for use in preparation of single cell suspension for sequencing.

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

hcodes

H334

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

H3506-VAR: + H3506-25G: + H3506-100MG: + H3506-5G: + H3506-1G: + H3506-500MG: + H3506-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
0000535110
0000535111
0000535109
0000535112
0000530903

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Heikki J Nieminen et al.
Scientific reports, 7(1), 3991-3991 (2017-06-24)
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A SAGE (serial analysis of gene expression) view of breast tumor progression.
Porter DA et al.
Cancer Research, 61, 5697-5697 (2001)
Highly efficient gene transfer into adult ventricular myocytes by recombinant adenovirus.
Kirshenbaum LA et al.
The Journal of Clinical Investigation, 92, 381-381 (1993)
Haoheng Yan et al.
The American journal of pathology, 176(3), 1421-1432 (2010-01-30)
Mutation and loss of function in p53 are common features among human breast cancers. Here we use BALB/c-Trp53+/- mice as a model to examine the sequence of events leading to mammary tumors. Mammary gland proliferation rates were similar in both
Hongzheng Sun et al.
Biology of reproduction, 100(3), 601-617 (2018-09-29)
Methionine adenosyltransferase II (MAT2A) is essential to the synthesis of S-adenosylmethionine, a major methyl donor, from L-methionine and ATP. Upon fertilization, zygotic genome activation (ZGA) marks the period that transforms the genome from transcriptional quiescence to robust transcriptional activity. During
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Articles

MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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Product Information sheet - H3506
Complex Carbohydrate Analysis- Enzymes, Kits and Reagents

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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