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H5505

Histone from calf thymus

Type III-S

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About This Item

CAS Number:
9064-47-5
UNSPSC Code:
41106305
NACRES:
NA.51
Beilstein/REAXYS Number:
1895465
MDL number:
MFCD00131329
Form:
powder
Biological source:
bovine (calf) thymus

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Product Name

Histone from calf thymus, Type III-S

biological source

bovine (calf) thymus

type

Type III-S

form

powder

concentration

≥60% protein (biuret)

technique(s)

activity assay: suitable

solubility

H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow

storage temp.

2-8°C

Quality Level

200

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Application

Histone is suitable for use:
  • as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein
  • in Cdk5 activity assay
  • in histone H1 kinase assay
  • as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity
  • as an acceptor of 32P from [γ-32P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells

Biochem/physiol Actions

Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.

Features and Benefits

Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.

Other Notes

Characterized as mainly subgroup f1 by SDS-PAGE

Preparation Note

Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

H5505-VAR: + H5505-100MG: + H5505-250MG: + H5505-BULK: + H5505-25MG:

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Certificates of Analysis (COA)

Lot/Batch Number
0000474415
0000474415
0000475742
0000475740
0000475740

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P Balsas et al.
Journal of hematology & oncology, 10(1), 80-80 (2017-04-01)
Pharmacological inhibition of B cell receptor (BCR) signaling has recently emerged as an effective approach in a wide range of B lymphoid neoplasms. However, despite promising clinical activity of the first Bruton's kinase (Btk) and spleen tyrosine kinase (Syk) inhibitors
Amit K Dutta et al.
Journal of colloid and interface science, 324(1-2), 55-60 (2008-05-30)
The real-time changes in viscoelasticity of adsorbed poly(L-lysine) (PLL) and adsorbed histone (lysine rich fraction) due to cross-linking by glutaraldehyde and corresponding release of associated water were investigated using a quartz crystal microbalance with dissipation monitoring (QCM-D) and attenuated total
Mária Čížková et al.
Cells, 8(7) (2019-07-20)
DNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter
Scott T
Concise encyclopedia of biochemistry, 265-265 (1988)
Liang Zheng et al.
Haematologica, 105(4), 1107-1119 (2019-11-23)
Thrombotic thrombocytopenic purpura (TTP) is caused by severe deficiency of ADAMTS13 (A13), a plasma metalloprotease that cleaves endothelium-derived von Willebrand factor (VWF). However, severe A13 deficiency alone is often not sufficient to cause an acute TTP; additional factors may be
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