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Merck

I3642

Monoclonal Anti-Interleukin-1β antibody produced in mouse

clone 8516.311, purified immunoglobulin, lyophilized powder

Synonym(s):

Anti-IL-1β

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About This Item

UNSPSC Code:
51111800
NACRES:
NA.41
MDL number:
MFCD00162514
Conjugate:
unconjugated
Clone:
8516.311, monoclonal
Application:
FACS, IHC, Neutral, WB
Citations:
7

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8516.311, monoclonal

form

lyophilized powder

species reactivity

human

technique(s)

flow cytometry: suitable, immunohistochemistry: 8-25 μg/mL using human PBMC′s, neutralization: suitable, western blot: 1 μg/mL

isotype

IgG1

UniProt accession no.

P01584

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... IL1B(3553)

Related Categories

Immunogen

purifed, E. coli-derived, recombinant human IL-1β.

Application

May used in immunoblotting, immunohistochemistry, ELISA, and neutralization assays

Biochem/physiol Actions

The antibody will neutralize the biological activity of recombinant human IL-1β. It will not neutralize the activity of recombinant human IL-α, recombinant mouse IL-1α, or recombinant mouse IL-1β. When immobilized on a microplate, this antibody will capture both recombinant as well as natural human IL-1β. In immunoblotting, the antibody shows ~25% cross-reactivity with recombinant rat IL-1β and <5% cross-reactivity with recombinant mouse IL-1β and recombinant porcine IL-1β. Additionally, in immunoblotting, the antibody exhibits no cross-reactivity with recombinant mouse IL-α, recombinant human IL-1ra, recombinant mouse IL-1ra, or recombinant rat IL-1α.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Preparation Note

Monoclonal Anti-Interleukin-1(IL-1) (mouse IgG1 isotype) is purified from a mouse hybridoma utilizing Protein A affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

I3642-BULK: + I3642-VAR: + I3642-.5MG: + I3642-.5MG-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
0000340371
0000323792
0000340371
0000323792
0000278609

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Melanie Genoula et al.
Frontiers in immunology, 9, 459-459 (2018-03-30)
The ability of Mycobacterium tuberculosis (Mtb) to persist in its human host relies on numerous immune evasion strategies, such as the deregulation of the lipid metabolism leading to the formation of foamy macrophages (FM). Yet, the specific host factors leading
Paul A Cordell et al.
Blood, 115(13), 2674-2681 (2010-01-21)
Factor XIII-A (FXIII-A) is present in the cytosol of platelets, megakaryocytes, monocytes, osteoblasts, and macrophages and may be released from cells by a nonclassical pathway. We observed that plasma FXIII-A levels were unchanged in thrombocytopenic mice (Bcl-x(Plt20/Plt20) and Mpl(-/-)), which
Ludmila Alekseeva et al.
BMC microbiology, 9, 33-33 (2009-02-12)
Aspergillus fumigatus, a saprophytic mould, is responsible for life-threatening, invasive pulmonary diseases in immunocompromised hosts. The role of the airway epithelium involves a complex interaction with the inhaled pathogen. Antimicrobial peptides with direct antifungal and chemotactic activities may boost antifungal
Mariángeles Clauzure et al.
Immunology, 163(4), 493-511 (2021-04-10)
The impairment of the cystic fibrosis transmembrane conductance regulator (CFTR) activity induces intracellular chloride (Cl- ) accumulation. The anion Cl- , acting as a second messenger, stimulates the secretion of interleukin-1β (IL-1β), which starts an autocrine positive feedback loop. Here
Tobias Wiedemann et al.
Gut, 61(7), 986-996 (2012-01-31)
One of the most important hormones in the human stomach is the peptide gastrin. It is mainly required for the regulation of gastric pH but is also involved in growth and differentiation of gastric epithelial cells. In Helicobacter pylori infected
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