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Merck

I7633

IMDM

With ʟ-glutamine and HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217469

Key Documents

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Product Name

Iscove′s Modified Dulbecco′s Medium, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

components

phenol red: yes
glucose: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

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Application

Iscove′s Modified Dulbecco′s Medium has been used to culture KG1a cells (human leukemic cell line) and Chinese hamster ovary (CHO) DG44 cell line.
Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

General description

Iscove′s Modified Dulbecco′s medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids, and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate. Iscove′s medium supports murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Preparation Note

Formulated to contain 17.7 grams of powder per liter of medium.
Supplement with 3.024 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

I7633-50L: + I7633-1L-KC: + I7633-25K: + I7633-50K: + I7633-PROC: + I7633-10L: + I7633-50KG: + I7633-VAR: + I7633-10X1L: + I7633-25KG: + I7633LF1-BIBC: + I7633-BIBC: + I7633-2X5L: + I7633-1L: + I7633-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
0000500463
0000500465
0000500465
0000500463
0000500460

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In order to establish an easy and quick construction method for obtaining a stable and highly productive gene-amplified recombinant Chinese Hamster Ovary (CHO) cell line, variouskinds of stepwise methotrexate (MTX) selection were carriedout. The specific growth and production rates of
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