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L5158

Leukemia Inhibitory Factor from mouse

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

Synonym(s):

Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, mLIF, LIF

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00283681
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
buffered aqueous solution

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Product Name

Leukemia Inhibitory Factor from mouse, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

buffered aqueous solution

quality

endotoxin tested

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

Quality Level

200

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Application

Leukemia Inhibitory Factor from mouse has been used:
  • as a component of enriched co-culture media
  • as a supplement in Dulbecco′s modified eagle′s medium (DMEM)/F12 medium for culturing heart cells
  • as a component in maintenance medium for maintaining embryonic stem cells
  • to treat ovaries in ovarian culture

Biochem/physiol Actions

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.
Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein that inhibits the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Physical form

Solution in phosphate buffered saline containing 0.02% TWEEN® 20

Analysis Note

The proliferative activity of mouse LIF is measured in culture using the M1 murine cell line.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

L5158-10UG: + L5158-VAR: + L5158-5UG-PW: + L5158-BULK: + L5158-5UG: + L5158-10UG-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
0000296349
0000296351
0000296351
0000296349
0000145912

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C-Kit Positive Cardiac Stem Cells and Bone Marrow-Derived Mesenchymal Stem Cells Synergistically Enhance Angiogenesis and Improve Cardiac Function After Myocardial Infarction in a Paracrine Manner
Bao L, et al.
Journal of Cardiac Failure, 23(5), 403-415 (2017)
Renée F de Pooter et al.
Methods in molecular biology (Clifton, N.J.), 330, 113-121 (2006-07-19)
Mature hematopoietic cells, like all other terminally differentiated lineages, arise during ontogeny via a series of increasingly restricted intermediates. Hematopoietic progenitors have their origin in the mesoderm, which gives rise to hemangioblasts that can differentiate into endothelial or endocardial precursors
Cristian Soitu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(23), 2001854-2001854 (2020-12-12)
There is an unmet demand for microfluidics in biomedicine. This paper describes contactless fabrication of microfluidic circuits on standard Petri dishes using just a dispensing needle, syringe pump, three-way traverse, cell-culture media, and an immiscible fluorocarbon (FC40). A submerged microjet
Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial follicle activation
Sutherland JM, et al.
Journal of Cellular Physiology, 227(3), 1188-1198 (2012)
Renée F de Pooter et al.
Methods in molecular biology (Clifton, N.J.), 380, 73-81 (2007-09-19)
Mature hematopoietic cells, like all other terminally differentiated lineages, arise during ontogeny via a series of increasingly restricted intermediates. Hematopoietic progenitors derive from the mesoderm, which gives rise to hemangioblasts that can differentiate into endothelial or endocardial precursors, or hematopoietic
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Articles

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

Related Content

Data Sheet - L5158

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