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Merck

L5520

L-15 Medium (Leibovitz)

Without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Leibovitz L15

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217482

Key Documents

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sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: 0.011 g/L
sodium pyruvate: 0.55 g/L

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

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General description

L-15 Medium (Leibovitz) was initially prepared to be used in carbon dioxide (CO2) free systems, which involves sodium bicarbonate. The complement of free base amino acids, salts, and galactose buffers it. This aids the maintenance of physiological pH control.

Application

L-15 Medium (Leibovitz) has been used:  
  • in protein incorporation for incubation of high blastula embryos and to avoid the abnormal development of embryos
  • as a supplement in sterile cell media for the dissection and isolation of kidney cells
  • as a component of culture buffer for the tissue culture of Newt lung cells

Preparation Note

Supplement with 0.3 g/L L-glutamine.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

L5520-100ML: + L5520-BULK: + L5520-PROC: + L5520-10L: + L5520-1L: + L5520PROC: + L5520-6X500ML: + L5520-PH: + L5520-VAR: + L5520-500ML: + L5520-24X500ML:

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Certificates of Analysis (COA)

Lot/Batch Number
0000426113
0000426113
0000364988
0000364988
0000353578

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