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Merck

L7759

Lectin from Arachis hypogaea (peanut)

peroxidase conjugate, lyophilized powder

Synonym(s):

PNA, Peanut agglutinin

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About This Item

NACRES:
NA.54
UNSPSC Code:
12352202
Form:
lyophilized powder
Biological source:
Arachis hypogaea (peanut)

Key Documents

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biological source

Arachis hypogaea (peanut)

conjugate

peroxidase conjugate

form

lyophilized powder

peroxidase activity

≥20 units/mg protein

technique(s)

western blot: suitable

storage temp.

−20°C

Quality Level

200

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Application

General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.

Physical form

Contains sodium citrate

Preparation Note

Prepared from peroxidase (P8375) using a modification of a published method, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Other Notes

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

L7759-.5MG: + L7759-VAR: + L7759-250UG: + L7759-1MG: + L7759-250UG-PW: + L7759-BULK: + L7759-1MG-PW:

jan

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Certificates of Analysis (COA)

Lot/Batch Number
0000523666
0000523665
0000523664
0000523663
0000523666

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Matthew R Kudelka et al.
Nature methods, 13(1), 81-86 (2015-12-01)
Protein O-glycosylation has key roles in many biological processes, but the repertoire of O-glycans synthesized by cells is difficult to determine. Here we describe an approach termed Cellular O-Glycome Reporter/Amplification (CORA), a sensitive method used to amplify and profile mucin-type
Ting-Hsuan Chen et al.
Methods in molecular biology (Clifton, N.J.), 2248, 139-153 (2020-11-14)
Virus-like particle (VLP) technology is an alternative platform for developing vaccines to combat seasonal and pandemic influenza. Influenza VLPs are non-infectious nanoparticles that can elicit effective vaccine immunogenicity in hosts. B-cell-activating factor (BAFF, or BLyS) and a proliferation-inducing ligand (APRIL)
Ritva Rice et al.
Scientific reports, 7(1), 12063-12063 (2017-09-25)
Ectothermal reptiles have internal pigmentation, which is not seen in endothermal birds and mammals. Here we show that the development of the dorsal neural tube-derived melanoblasts in turtle Trachemys scripta is regulated by similar mechanisms as in other amniotes, but
Jessica Tan et al.
Journal of virology, 96(2), e0142121-e0142121 (2021-10-21)
The public health burden caused by influenza virus infections is not adequately addressed with existing vaccines and antivirals. Identifying approaches that interfere with human-to-human transmission of influenza viruses remains a pressing need. The importance of neuraminidase (NA) activity for the
Anna Iervolino et al.
PloS one, 10(3), e0119142-e0119142 (2015-03-24)
Dicer is a crucial enzyme for the maturation of miRNAs. Mutations in the Dicer gene are highly associated with Pleuro Pulmonary Blastoma-Family Dysplasia Syndrome (PPB-FDS, OMIM 601200), recently proposed to be renamed Dicer syndrome. Aside from the pulmonary phenotype (blastoma)
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Product Information Sheet - L7759

Product Information Sheet

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