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M3024

Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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Properties

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

Earle’s salts (5% CO₂): yes
L-glutamine: no
HEPES: no
NaHCO₃: no
sodium pyruvate: no
phenol red: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used:

to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
to culture MCF-7 cells for experimental treatments with hormone
to maintain zebrafish pineal glands for action spectrum analysis

Features and Benefits

Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells

Preparation Note

Formulated to contain 9.3 grams of powder per liter of medium.

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

M3024-10L:4.548173395791E12

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Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.