MEM Vitamin Solution (100×)

MEM Vitamin Solution (100×) is a sterile-filtered bioreagent, which is suitable for cell culture.

Synthetic/ basal media used currently contain amino acids, carbohydrates, vitamins and salts. Eagle′s minimum essential medium (MEM) is a modified version of BME (basal medium Eagle′s), which contains increased amounts of each amino acid. Addition of water soluble vitamins such as, inositol to various culture facilitates the hatching of hamster and rabbit blastocysts. Vitamins also influence the metabolism of glucose in sheep and mouse embryos.

MEM Vitamin Solution (100×) has been used in:

• glial conditioned medium for primary neuronal culture
• in Luria-Bertani (LB) medium or minimal medium (MM)
• in private medium (PM = aminoacid-starved medium) for the Aa deprivation experiments

MEM Vitamin Solution (100×) is used for the following applications:

• In the preparation of DMEM medium (for primary neural cell culture)
• For the maintenance of cell lines in Eagle′s Minimum Essential Medium (the medium is supplemented with MEM Vitamin Solution along with other components)

Adding supplements of vitamins to media stimulates growth and prolongs the viability of cells in culture. This concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.