MAK113
ATPase/GTPase Activity Assay Kit
sufficient for 200 colorimetric tests
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 200 colorimetric tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
activity assay: suitable
input
liquid
detection method
colorimetric
relevant disease(s)
cancer
storage temp.
2-8°C
Related Categories
General description
ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation.
Application
ATPase/GTPase Activity Assay Kit has been used to measure the ATPase enzyme activity in:
- Dicer-like 1 (DCL1) and its mutants, to terminate the reaction and generate a colorimetric product
- various brain regions of mice, facilitating the quantification of inorganic phosphate released from ATP
- cell lysates, for the development and encapsulation of the reconstituted cell-free enzyme system
Suitable for the detection of ATPase and GTPase activity and for the screening of ATPase and GTPase inhibitors.
Biochem/physiol Actions
The ATPase/GTPase Activity Assay kit provides a simple and direct procedure for measuring ATPase/GTPase activity in a microplate format. This kit uses a single reagent formulation to accurately determine enzyme activity in 30 minutes at room temperature. The malachite green reagent forms a stable dark green color with free phosphate liberated by the enzymes resulting in a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme activity present One unit of activity is the amount of enzyme that catalyzes the production of 1 μmole of free phosphate per minute under the assay conditions.
Features and Benefits
Compatible with high-throughput handling systems.
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 2
Storage Class
12 - Non Combustible Liquids
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Please refer to KIT Component information
pdsc
Please refer to KIT Component information
prtr
Please refer to KIT Component information
fsl
Please refer to KIT Component information
ishl_indicated
Please refer to KIT Component information
ishl_notified
Please refer to KIT Component information
cart
キットコンポーネントの情報を参照してください
jan
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Meng-Yan Sun et al.
Oncology reports, 39(6), 2673-2680 (2018-04-06)
Regulator of G‑protein signaling 1 (RGS1) has been found to be a critical factor in melanoma and other malignancies. However, the mechanism involved in the RGS1‑mediated promotion of melanoma progression is not clear. We based our study on samples collected from
Shuhui Wang et al.
Protein & cell, 11(2), 124-137 (2019-11-24)
Type VII secretion systems (T7SSs) are found in many disease related bacteria including Mycobacterium tuberculosis (Mtb). ESX-1 [early secreted antigen 6 kilodaltons (ESAT-6) system 1] is one of the five subtypes (ESX-1~5) of T7SSs in Mtb, where it delivers virulence
Senyuan Cai et al.
Bioorganic & medicinal chemistry, 27(7), 1274-1282 (2019-02-23)
Filamenting temperature-sensitive mutant Z (FtsZ) is recognized as a promising target for new antibiotics development because of its high conservatism and pivotal role in the bacteria cell division. The aromatic heterocyclic scaffold of indole is known showing merit medical functions
Buqing Ye et al.
Nature communications, 8(1), 1518-1518 (2017-11-16)
Lymphoid lineage commitment is an important process in haematopoiesis, which forms the immune system to protect the host from pathogen invasion. However, how multipotent progenitors (MPP) switch into common lymphoid progenitors (CLP) or common myeloid progenitors (CMP) during this process
Meifang Li et al.
International journal of biological sciences, 15(12), 2522-2537 (2019-11-23)
Despite remarkable advancements in our understanding of breast cancer, it remains the leading cause of cancer deaths in women. Distant recurrence and metastasis is the main reason for death due to breast cancer. It is well recognized that the GATA
Related Content
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service