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Merck

N1627

4-Nitrophenyl β-D-glucuronide

≥98% (TLC)

Synonym(s):

4-Nitrophenyl β-D-glucuronide, 4-Nitrophenyl-β-D-glucopyranosiduronic acid, PNPG

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About This Item

Empirical Formula (Hill Notation):
C12H13NO9
CAS Number:
10344-94-2
Molecular Weight:
315.23
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
24897507
EC Number:
233-753-0
Beilstein/REAXYS Number:
1438576
MDL number:
MFCD00036210

Key Documents

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InChI key

QSUILVWOWLUOEU-GOVZDWNOSA-N

InChI

1S/C12H13NO9/c14-7-8(15)10(11(17)18)22-12(9(7)16)21-6-3-1-5(2-4-6)13(19)20/h1-4,7-10,12,14-16H,(H,17,18)/t7-,8-,9+,10-,12+/m0/s1

SMILES string

O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)Oc2ccc(cc2)[N+]([O-])=O

grade

Molecular Biology

assay

≥98% (TLC)

form

powder

solubility

H2O: soluble 100 mg/mL, clear

storage temp.

−20°C

Quality Level

200

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General description

Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Application

4-Nitrophenyl β-D-glucuronide has been used as substrate in acetate buffer to measure β-glucuronidase.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

N1627-50MG: + N1627-100MG: + N1627-BULK: + N1627-2G: + N1627-VAR: + N1627-250MG: + N1627-25MG: + N1627-1G:

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Certificates of Analysis (COA)

Lot/Batch Number
0000430784
0000430783
0000430783
0000430784
0000349435

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A tool for monitoring Trichoderma harzianum: II. The use of a GUS transformant for ecological studies in the rhizosphere
Green Helge and Jensen D Funck
Phytopathology, 85(11), 1436-1440 (1995)
beta-N-Cyanoethyl acyl hydrazide derivatives: A new class of beta-glucuronidase inhibitors
Khan KM, et al.
Chemical & Pharmaceutical Bulletin, 50(11), 1443-1446 (2002)
E S Piruzian et al.
Molecular genetics and genomics : MGG, 266(5), 778-786 (2002-01-26)
The coding region of the licB gene from Clostridium thermocellum was truncated at the 3' end. The modified lichenase encoded by the construct (LicBM2) retained the most important properties of the enzyme - its high activity and thermostability. LicBM2 consists
Analysis of Fluorescent Compounds in Urine by Liquid Chromatography
T. Hanaia & J. Huberta
Journal of Liquid Chromatography, 7, 1627-1642 (1984)
Rapid separation of p-nitrophenol and its glucuronide and sulfate conjugates by reversed-phase high-performance liquid chromatography.
G Diamond et al.
Journal of chromatography, 177(2), 368-371 (1979-09-21)
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