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N2513

Nerve Growth Factor β rat

≥97% (SDS-PAGE), recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(s):

NGF-β from rat, Nerve Growth Factor-β from rat

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About This Item

CAS Number:
86923-98-0
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00164982
Biological source:
rat
Recombinant:
expressed in Sf21 cells
Assay:
≥97% (SDS-PAGE)
Form:
lyophilized powder
Impurities:
endotoxin, tested

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Product Name

NGF-β from rat, recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture

biological source

rat

recombinant

expressed in Sf21 cells

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.15-3.00 ng/mL EC50

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

solubility

water: soluble 0.1 g/L at 20 °C

UniProt accession no.

P25427

storage temp.

−20°C

Quality Level

200

Gene Information

rat ... NGF(4803)

Related Categories

General description

NGFβ (nerve growth factor β) is a protein involved in signal transduction, and expressed by multiple tissues such as placenta, urothelium and bladder smooth muscle cells. It is a neurotrophin and belongs to the NGF family.

Application

NGF-β from rat is used for the following applications:
  • In the preparation of DMEM medium (for the cultivation and differentiation of PC12 cells)
  • Western blotting analysis
  • For the preparation of differentiation medium (it is prepared from DMEM, in which NGF-β is one of the components)
  • In cell culture and treatment (for neuronal differentiation)
  • In the preparation of stock solution

Biochem/physiol Actions

NGFβ (nerve growth factor β) is essential for the differentiation and survival of neurons. It also plays a key role in the development and maintenance of nervous system. It is also involved in angiogenesis, and facilitates tumorigenesis such as, oral, breast and prostate cancers. It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB), and might also aid in determining the therapy for the same. It might be involved in changes in placental function, as NGF signaling is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS and 1 M NaCl containing 50 μg of bovine serum albumin per 1 μg of cytokine

Analysis Note

The biological activity is measured in a cell proliferation assay using the factor-dependent human erythroleukemic cell line, TF-1.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act Listed

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N2513-BULK: + N2513-.1MG: + N2513-.1MG-PW: + N2513-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000534986
0000534986
0000480832
0000480832
0000426700

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Zhe Gong et al.
Journal of neuroinflammation, 15(1), 242-242 (2018-08-30)
Nod-like receptor protein 3 (NLRP3) inflammasome is a crucial factor in mediating inflammatory responses after cerebral ischemia/reperfusion (I/R), but the cellular location of NLRP3 inflammasome in cerebral I/R has yet come to a conclusion, and there is still no specific
A Khodorova et al.
Neuroscience, 254, 312-323 (2013-10-08)
Nerve growth factor (NGF) augments the excitability of isolated rat sensory neurons through activation of the p75 neurotrophin receptor (p75(NTR)) and its downstream sphingomyelin signaling cascade, wherein neutral sphingomyelinase(s) (nSMase), ceramide, and the atypical protein-kinase C (aPKC), protein-kinase M zeta
Guangyi Zhao et al.
Molecular medicine reports, 16(4), 4678-4684 (2017-08-30)
The present study aimed to investigate the effect of exogenous nerve growth factor (NGF) pretreatment on demyelination in the spinal cord of lidocaine‑treated rats, and explored the potential neuroprotective mechanisms of NGF. A total of 36 rats were randomly assigned
Xiao-Ping Huang et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 89, 124-134 (2017-02-22)
The aim of this study was to explore the effect by which the combination of Astragaloside IV (AST IV) and Ginsenoside Rg1 (Rg1) resisted autophagic injury in PC12 cells induced by oxygen glucose deprivation/reoxygenation (OGD/R). We studied the nature of
Hong-Chen Qu et al.
PloS one, 9(9), e106321-e106321 (2014-09-03)
To examine whether urinary nerve growth factor (NGF) could serve as a biomarker for interstitial cystitis/painful bladder syndrome (IC/PBS), we conducted a comprehensive meta-analysis of 9 studies. Among the studies considered, patients with IC/PBS had higher urinary NGF and NGF/Cr
View All Related Papers

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Data Sheet - N2513

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