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Merck

N3910

Neutralization Solution B

Synonym(s):

DNA neutralization solution, neutralization buffer DNA extraction

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About This Item

NACRES:
NA.25
UNSPSC Code:
12161703

Key Documents

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grade

Molecular Biology

form

liquid

Quality Level

200

feature

Direct PCR

technique(s)

DNA amplification: suitable, DNA extraction: suitable, PCR: suitable

input

crude DNA

storage temp.

−20°C

General description

This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.

Application

Neutralization Solution B has been used in the modified Sigma Extract-N-Amp™ extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp™ PCR Reaction Mix.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

N3910-60ML: + N3910-2.5ML-KC: + N3910-240ML: + N3910-6ML-KC: + N3910-24ML: + N3910-2.5ML: + N3910-1.2ML: + N3910-240ML-KC: + N3910-VAR: + N3910-1.2ML-KC: + N3910-60ML-KC: + N3910PROC: + N3910-BULK: + N3910-24ML-KC: + N3910-6ML:

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Certificates of Analysis (COA)

Lot/Batch Number
0000470472
0000470472
0000318607
0000381622
0000381620

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Işın Çakır et al.
Nature metabolism, 4(1), 44-59 (2022-01-19)
The adipose tissue-derived hormone leptin can drive decreases in food intake while increasing energy expenditure. In diet-induced obesity, circulating leptin levels rise proportionally to adiposity. Despite this hyperleptinemia, rodents and humans with obesity maintain increased adiposity and are resistant to
Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
Jun He et al.
Pharmacology, biochemistry, and behavior, 86(2), 327-333 (2006-12-16)
Adolescence is an important stage of brain development. Recent studies have indicated that neurogenesis in the brain occurs throughout life prompting comparisons of adolescent and adult neurogenesis. Since insulin-like growth factor 1 (IGF-1) has been implicated in promoting neurogenesis we
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Protocols

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

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