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Merck

N6013

Nutrient Mixture F-10 Ham

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Ham’s F-10

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217607

Key Documents

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Product Name

Nutrient Mixture F-10 Ham, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

HEPES: no
phenol red: yes
L-glutamine: no
sodium pyruvate: 0.11 g/L
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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General description

F-10 Ham supports the growth of human diploid cells, white blood cells for chromosomal analysis, and primary explants of rat, rabbit, and chicken tissues. Ham′s Nutrient Mixtures are developed to support the clonal growth of several clones like Chinese hamster ovary (CHO) cells, clones of HeLa, and mouse L-cells.

Application

Nutrient Mixture F-10 Ham has been used:

  • to culture lamina cribrosa explants
  • in in vitro culture of lizard tail regenerate
  • for culturing of lizard embryo-fibroblasts

Preparation Note

Supplement with 0.146 g/L L-glutamine.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

N6013-10L: + N6013-500ML: + N6013-6X500ML: + N6013-BULK: + N6013-PROC: + N6013-100ML: + N6013PROC: + N6013-VAR: + N6013-PH:

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL8372
RNBL2670
RNBK4871
RNBJ2799
RNBJ2798

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Elisabetta Polazzi et al.
Tissue & cell, 43(6), 350-358 (2011-08-30)
An in vitro system of isolated skin cells has been developed in order to address the understanding on the factors that control the shedding cycle and differentiation of lizard epidermis. The skin from the regenerating lizard tail has been separated
Navita N Lopez et al.
Experimental eye research, 197, 108103-108103 (2020-06-12)
The lamina cribrosa is the initial site of glaucomatous injury. Pathological changes to the lamina cribrosa include posterior displacement of the lamina cribrosa, loss of trophic support, and remodeling of the extracellular matrix. Optic nerve head (ONH) astrocytes and lamina
Roser Buscà et al.
BMC evolutionary biology, 15, 179-179 (2015-09-04)
The Ras/Raf/MEK/ERK signaling pathway is involved in essential cell processes and it is abnormally activated in ~30 % of cancers and cognitive disorders. Two ERK isoforms have been described, ERK1 and ERK2; ERK2 being regarded by many as essential due

Articles

Nutrient Mixture F-10 Ham Formulation

Nutrient Mixture F-10 Ham Formulation

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