P1585
PMA
for use in molecular biology applications, ≥99% (HPLC), Molecular Biology
Synonym(s):
Phorbol 12-myristate 13-acetate, NF-κB Activator, PKC Activator, croton oil, phorbol ester, protein kinase C activator, 12-O-Tetradecanoylphorbol 13-acetate, 4β,9α,12β,13α,20-Pentahydroxytiglia-1,6-dien-3-one 12-tetradecanoate 13-acetate, PMA, TPA
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About This Item
InChI
1S/C36H56O8/c1-7-8-9-10-11-12-13-14-15-16-17-18-29(39)43-32-24(3)35(42)27(30-33(5,6)36(30,32)44-25(4)38)20-26(22-37)21-34(41)28(35)19-23(2)31(34)40/h19-20,24,27-28,30,32,37,41-42H,7-18,21-22H2,1-6H3/t24-,27+,28-,30-,32-,34-,35-,36-/m1/s1
SMILES string
CCCCCCCCCCCCCC(=O)O[C@@H]1[C@@H](C)[C@@]2(O)C(C=C(CO)C[C@@]3(O)C2C=C(C)C3=O)C4C(C)(C)[C@]14OC(C)=O
InChI key
PHEDXBVPIONUQT-RGYGYFBISA-N
grade
Molecular Biology
assay
≥99% (HPLC)
form
(Lyophilisate)
mol wt
616.83 g/mol
storage condition
protect from light
solubility
DMSO: soluble (DMSO solutions can be stored dark at −20 °C for at least six months.), ethanol: soluble (Dissolve in water-miscible organic solvent prior to preparing aqueous solutions)
storage temp.
−20°C
Quality Level
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General description
Through the protein kinase C (PKC) signaling pathway, PMA is a tumor promoter associated with:
- gene transcription
- cell growth
- cell differentiation
- programmed cell death
- immune pathway
- receptor desensitization
Phorbol 12-myristate 13-acetate is used to study:
- growth factors
- hormones
- cytokines-mediated regulation of cellular growth and differentiation
Application
- for the detection of intracellular cytokines
- in CellROX oxidative stress assay
- for Huh7 cell stimulation to investigate dipeptidyl peptidase 9 (DPP9) re-distribution
- as a positive control in B-cell receptor (BCR) stimulation of chronic lymphocytic leukaemia (CLL) cells
- in the differentiation of monocytes (THP -1) into macrophages
- molecular biology studies as standard
- analytical studies of tumor promoters
- promotion of cell growth
Biochem/physiol Actions
Features and Benefits
- Stimulation of interleukin 2 production of Jurkat cells
- T-cell stimulation
- Protein kinase C (PKC) activator
- NF-κB Activator
- Promotion of cell growth
- Used in analytical studies of tumor promoters
- Molecular Biology grade
Analysis Note
- Stimulation of interleukin 2 production of Jurkat cells
- T-cell stimulation
- Protein kinase C (PKC) activator
- NF-κB Activator
- Promotion of cell growth
- Used in analytical studies of tumor promoters
- Molecular Biology grade
Disclaimer
signalword
Danger
Hazard Classifications
Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
P1585-1MG-PW: + P1585-25MG-PW: + P1585-10MG-PW: + P1585-25MG: + P1585-5MG-PW: + P1585-BULK: + P1585-1MG: + P1585-VAR: + P1585-10MG: + P1585-5MG:
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Related Content
T-cell activation is normally triggered by the interaction of a cell surface receptor to its specific ligand molecule
PMA, through its activation of PKC, can activate T-cells and stimulate low-level production of IL-2. When Jurkat cells are stimulated by PMA and a co-stimulator, such as PHA, IL-2 production is strongly enhanced2.
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