Skip to Content
Merck

P1860

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, DMSO solution

Synonym(s):

Protease Inhibitor Solution, protease inhibitor

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
EC Number:
200-664-3
MDL number:
MFCD00677817
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution

form

DMSO solution

solubility

water: soluble

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.

Application

Protease Inhibitor Cocktail has been used:
  • as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
  • to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
  • in trypsin solution for trypsin-inhibited studies
  • as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot

This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.

Biochem/physiol Actions

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases
Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.

Features and Benefits

  • Broad specificity
  • Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
  • Contains no metal chelators: Ensures compatibility with downstream applications
  • Convenient packaging: 1 mL in glass bottle
  • Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Physical form

Solution in DMSO (D 2650, Hybri-Max).

Preparation Note

Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Other Notes

Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Disclaimer

After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

188.6 °F

flash_point_c

87 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Group 4: Flammable liquids + Type 3 petroleums + Hazardous rank III + Water insoluble liquid

fsl

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

P1860-BULK: + P1860-1ML: + P1860-1ML-PW: + P1860-VAR:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000440367
0000440367
0000327277
0000327277
0000198327

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

TMEM16A/ANO1 calcium-activated chloride channel as a novel target for the treatment of human respiratory syncytial virus infection
Pearson H, et al.
Thorax, 76(1), 64-72 (2021)
Fabry disease in the Spanish population: observational study with detection of 77 patients
Vieitez I, et al.
Orphanet Journal of Rare Diseases, 13, 1-13 (2018)
Matthew Townsend et al.
The Journal of physiology, 572(Pt 2), 477-492 (2006-02-14)
The accumulation of amyloid beta-protein (Abeta) in brain regions serving memory and cognition is a central pathogenic feature of Alzheimer's disease (AD). We have shown that small soluble oligomers of human Abeta that are naturally secreted by cultured cells inhibit
Takuma Shiratori et al.
Journal of immunology (Baltimore, Md. : 1950), 200(1), 218-228 (2017-11-17)
As osteoclasts have the central roles in normal bone remodeling, it is ideal to regulate only the osteoclasts performing pathological bone destruction without affecting normal osteoclasts. Based on a hypothesis that pathological osteoclasts form under the pathological microenvironment of the
Asja Guzman et al.
Biomaterials, 115, 19-29 (2016-11-24)
Invasive breast cancer and other tumors of epithelial origin must breach a layer of basement membrane (BM) that surrounds the primary tumor before invading into the adjacent extracellular matrix. To analyze invasive strategies of breast cancer cells during BM breaching
View All Related Papers

Articles

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

Related Content

プロテアーゼ阻害剤：サンプル調製中にタンパク質が失われないように

タンパク質の単離と特性解析におけるタンパク質分解を予防して、サンプル調製でタンパク質を保護するために、ニーズに応じてさまざまなプロテアーゼ阻害剤を選択しましょう。

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Data Sheet Japanese - P1860
Data Sheet - P1860

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service