P2645
Protein Kinase A Catalytic Subunit from bovine heart
≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)
Synonym(s):
PKA
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About This Item
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.32
MDL number:
Specific activity:
≥9 units/μg protein (cyclic-AMP is not required for this activity)
form
lyophilized (white powder to sticky mass to hard pellet)
specific activity
≥9 units/μg protein (cyclic-AMP is not required for this activity)
mol wt
40,862 Da
storage temp.
−20°C
Quality Level
General description
Protein Kinase A enzyme is composed of two subunits- catalytic and regulatory. The catalytic subunit exists as a monomer in the presence of cAMP and has a molecular weight of 40,862 Da.
Application
Protein Kinase A (PKA) Catalytic Subunit from bovine heart has been used-
- to study PKA-mediated inhibition of IRK1 (inwardly rectifying K+) channels
- in in Vitro PKA pPhosphorylation assay
- in Vitro affinity binding assays
- to study effects of PKA on inspiratory drive currents in functionally active motorneurons
Biochem/physiol Actions
Protein Kinase A (PKA) controls the transduction of Hedgehog signaling and participates in proliferation and fate specification. It phosphorylates several neurotransmitter receptors, transcription factors and constituents of various intracellular signaling pathways.
Protein Kinase A catalyzes the transfer of terminal phosphate from ATP to threonine or serine residues present on various proteins. This protein is inactive in the absence of cAMP, where the catalytic and regulatory subunits are bound together. The regulatory subunit, in the presence of cAMP, binds to cAMP and releases the catalytic subunit.
Packaging
Package size based on phosphorylating units
Physical form
Lyophilized powder with sucrose and phosphate buffer salts as stabilizer.
Preparation Note
Prepared from protein kinase A (P 5511)
Other Notes
Phosphorylating Activity: One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C, determined by measuring the production of ADP.
Disclaimer
Please note that the pack size has been changed to align with the unit definition, while the number of phosphorylating units remain the same as before.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
P2645-1000UN-PW: + P2645-12.5KU: + P2645-500UN: + P2645-PH: + P2645-400UN-PW: + P2645-1000UN: + P2645-400UN: + P2645-250UN: + P2645-2.5KU: + P2645-1KU: + P2645-BULK: + P2645-100UN: + P2645-VAR:
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Protein kinase A activity and Hedgehog signaling pathway
Vitamins & Hormones, 88, 273-291 (2012)
Petr G Vikhorev et al.
Cardiovascular research (2020-11-03)
Dilated cardiomyopathy (DCM) is associated with mutations in many genes encoding sarcomere proteins. Truncating mutations in the titin gene TTN are the most frequent. Proteomic and functional characterisations are required to elucidate the origin of the disease and the pathogenic
J A Byun et al.
Science advances, 6(25), eabb1250-eabb1250 (2020-07-01)
The functional response of a signaling system to an allosteric stimulus often depends on subcellular conditions, a phenomenon known as pluripotent allostery. For example, a single allosteric modulator, Rp-cAMPS, of the prototypical protein kinase A (PKA) switches from antagonist to
Regions of the Escherichia coli primary sigma factor sigma70 that are involved in interaction with RNA polymerase core enzyme.
Nagai, et al.
Genes Cells, 2, 725-734 (2019)
Christopher M Bocchiaro et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(4), 1099-1103 (2003-02-25)
Plasticity underlying adaptive, long-term changes in breathing behavior is hypothesized to be attributable to the modulation of respiratory motoneurons by intracellular second-messenger cascades. In quiescent preparations, protein kinases, including cAMP-dependent protein kinase A (PKA), potentiate glutamatergic inputs. However, the dynamic
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