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Merck

P2922

Endoproteinase Glu-C from Staphylococcus aureus V8

Type XVII-B, lyophilized powder, 500-1,000 units/mg solid

Synonym(s):

V8 Protease

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About This Item

CAS Number:
66676-43-5
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
EC Number:
3.4.21.19 (BRENDA, IUBMB)
MDL number:
MFCD01324998
Specific activity:
500-1,000 units/mg solid

Key Documents

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type

Type XVII-B

form

lyophilized powder

specific activity

500-1,000 units/mg solid

mol wt

29 kDa

purified by

chromatography

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

Gene Information

Staphylococcus aureus subsp. aureus MW2 ... sspA(1003044)

Related Categories

Application

Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . Product P2922 has been used to linearize cyclic peptides in C. ternatea leaf extract.
Endoproteinase Glu-C from Staphylococcus aureus V8 has been used to digest reduced and alkylated cyclotides to produce linearized fragments.
It is used for selective cleavage of proteins for amino acid sequence determination or peptide mapping.

Biochem/physiol Actions

Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.

Other Notes

One unit will hydrolyze 1 μmole of N-t-Boc-L-glutamic acid α-phenyl ester per min at pH 7.8 at 37 °C. One unit is equivalent to ~0.004 casein digestion unit.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P2922-PPG: + P2922-500UN: + P2922-2.5KU: + P2922-500UN-PW: + P2922-PH: + P2922-100UN: + P2922-100UN-PW: + P2922-BULK: + P2922-VAR: + P2922-2.5KU-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
0000472893
0000472893
0000477189
0000477189
0000472892

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Andrew Michael Frey et al.
Cell reports, 35(1), 108930-108930 (2021-04-08)
Staphylococcus aureus possesses ten extracellular proteases with mostly unknown targets in the human proteome. To assist with bacterial protease target discovery, we have applied and compared two N-terminomics methods to investigate cleavage of human serum proteins by S. aureus V8 protease
Shanshan Liu et al.
Amino acids, 48(4), 1059-1067 (2016-01-11)
Common yet often overlooked, deamidation of peptidyl asparagine (Asn or N) generates aspartic acid (Asp or D) or isoaspartic acid (isoAsp or isoD). Being a spontaneous, non-enzymatic protein post-translational modification, deamidation artifact can be easily introduced during sample preparation, especially
Hélio Nitta Matsuura
Methods in molecular biology (Clifton, N.J.), 2469, 165-181 (2022-05-05)
Cyclotides are small circular peptides carrying an array of interesting biological activities and also showing interesting features for storage and bioavailability. Here, an optimized method to isolate cyclotides from two species of Psychotria, P. brachyceras and P. leiocarpa, that can
Yurgis Av Yomantas et al.
Microbial cell factories, 10, 64-64 (2011-08-09)
Plasmid-less, engineered Bacillus strains have several advantages over plasmid-carrier variants. Specifically, their stability and potential ecological safety make them of use in industrial applications. As a rule, however, it is necessary to incorporate many copies of a key gene into
From the Cover: Discovery of an unusual biosynthetic origin for circular proteins in legumes
Aaron G. Poth, Michelle L. Colgrave, et al.
Proceedings of the National Academy of Sciences of the USA, 108 (2011)
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