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Merck

P3243

Phosphodiesterase I from Crotalus adamanteus venom

vial of ≥100 units, Purified

Synonym(s):

5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase

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About This Item

CAS Number:
9025-82-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-806-5
MDL number:
MFCD00131883
EC Number:
3.1.4.1 (BRENDA, IUBMB)
Specific activity:
≥20.0 unit/mg solid
Biological source:
Crotalus adamanteus venom

Key Documents

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biological source

Crotalus adamanteus venom

form

solid

quality

Purified

specific activity

≥20.0 unit/mg solid

packaging

vial of ≥100 units

storage temp.

−20°C

Quality Level

200

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Application

Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P3243 is from Crotalus adamanteus venom and is purified. Product P3243 has been used to hydrolyze tRNA with wyosine derivatives into mononucleosides.

Biochem/physiol Actions

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.

Other Notes

One Unit hydrolyzes one μmole of p-nitrophenyl thymidine-5-phosphate per minute at 25 °C, pH 8.9

Preparation Note

For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL.
Purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P3243-BULK: + P3243-VAR: + P3243-1VL: + P3243-1VL-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
0000491541
0000491541
0000486170
0000486170
0000484501

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Valérie de Crécy-Lagard et al.
Molecular biology and evolution, 27(9), 2062-2077 (2010-04-13)
Wyosine (imG) and its derivatives such as wybutosine (yW) are found at position 37 of phenylalanine-specific transfer RNA (tRNA(Phe)), 3' adjacent to the anticodon in Eucarya and Archaea. In Saccharomyces cerevisiae, formation of yW requires five enzymes acting in a
Action of venom phosphodiesterase on deoxyribonucleic acid.
E J WILLIAMS et al.
The Journal of biological chemistry, 236, 1130-1134 (1961-04-01)
N Shenoy et al.
Blood cancer journal, 7(7), e587-e587 (2017-07-22)
The Ten Eleven Translocation (TET) enzymes have been found to be mutated in both diffuse large B-cell (DLBCL) and peripheral T-cell (PTCL) lymphomas resulting in DNA hypermethylation. Recent studies in embryonal stem cells showed that ascorbic acid (AA) is a
Yana Konokhova et al.
Skeletal muscle, 6, 10-10 (2016-02-20)
Low mitochondrial content and oxidative capacity are well-established features of locomotor muscle dysfunction, a prevalent and debilitating systemic occurrence in patients with chronic obstructive pulmonary disease (COPD). Although the exact cause is not firmly established, physical inactivity and oxidative stress
Felix A Dingler et al.
Molecular cell, 80(6), 996-1012 (2020-11-05)
Reactive aldehydes arise as by-products of metabolism and are normally cleared by multiple families of enzymes. We find that mice lacking two aldehyde detoxifying enzymes, mitochondrial ALDH2 and cytoplasmic ADH5, have greatly shortened lifespans and develop leukemia. Hematopoiesis is disrupted
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Protocols

Enzymatic Assay of 5’-Nucleotidase

To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.

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