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Merck

P7130

Puromycin aminonucleoside

Synonym(s):

3′-Amino-3′-deoxy-N6,N6-dimethyladenosine

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About This Item

Empirical Formula (Hill Notation):
C12H18N6O3
CAS Number:
58-60-6
Molecular Weight:
294.31
UNSPSC Code:
51281912
NACRES:
NA.85
PubChem Substance ID:
24898823
EC Number:
200-388-3
Beilstein/REAXYS Number:
93902
MDL number:
MFCD00063462

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InChI key

RYSMHWILUNYBFW-VENHTOENSA-N

InChI

1S/C12H18N6O3/c1-17(2)10-8-11(15-4-14-10)18(5-16-8)12-9(20)7(13)6(3-19)21-12/h4-7,9,12,19-20H,3,13H2,1-2H3/t6-,7+,9+,12-/m1/s1

SMILES string

CN(C)c1ncnc2n(cnc12)[C@@H]3O[C@H](CO)[C@H](N)[C@@H]3O

form

powder

solubility

H2O: soluble 50 mg/mL

antibiotic activity spectrum

Gram-positive bacteria, neoplastics, parasites

mode of action

protein synthesis | interferes

storage temp.

2-8°C

Quality Level

300

Related Categories

General description

Puromycin aminonucleoside is the aminonucleoside portion of the antibiotic puromycin. It is useful in nephrology research, like studies of focal and segmental glomerulosclerosis and in the induction of nephrosis in rats. The excretion of sodium and nitrite (NOx) metabolites in rats with puromycin aminonucleoside-induced nephrotic syndrome are studied. Puromycin aminonucleoside-induced nephrosis in rats has been studied with respect to the production of reactive oxygen species during the acute phase. Puromycin aminonucleoside is used to probe endothelial glycosaminoglycan synthesis in cultured glomerular endothelial cells and their relation to cell permeability.

Application

Puromycin aminonucleoside has been used:
  • as a selection marker for the infected cells for the transfection of lentivirus
  • as a component of fresh medium for transfection and infection assays
  • to subcutaneously induce puromycin aminonucleoside nephrosis to investigate the mRNA and protein levels of nephrin and podocin before the onset of proteinuria

Biochem/physiol Actions

Puromycin aminonucleoside is used to study human glomerular disease by inducing damage of murine glomerular podocytes and is used to study glomerular function and morphology.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.

signalword

Warning

pictograms

Health hazard
GHS08

hcodes

H373

Hazard Classifications

STOT RE 2

target_organs

Kidney

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P7130-100MG-PW: + P7130-BULK: + P7130-1G-PW: + P7130-25MG: + P7130-500MG: + P7130-100MG: + P7130-VAR: + P7130-10MG: + P7130-1G: + P7130-500MG-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
0000436781
0000436781
0000445548
0000445548
0000445550

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Ramzi Khalil et al.
The Journal of pathology, 247(2), 177-185 (2018-10-24)
Dynamin plays an essential role in maintaining the structure and function of the glomerular filtration barrier. Specifically, dynamin regulates the actin cytoskeleton and the turnover of nephrin in podocytes, and knocking down dynamin expression causes proteinuria. Moreover, promoting dynamin oligomerization
Ido Refaeli et al.
Scientific reports, 10(1), 9419-9419 (2020-06-12)
Dominant and recessive mutations in podocalyxin (PODXL) are associated with human kidney disease. Interestingly, some PODXL mutations manifest as anuria while others are associated with proteinuric kidney disease. PODXL heterozygosity is associated with adult-onset kidney disease and podocalyxin shedding into
Anastasia Korolj et al.
Lab on a chip, 18(20), 3112-3128 (2018-09-29)
Most kidney diseases begin with abnormalities in glomerular podocytes, motivating the need for podocyte models to study pathophysiological mechanisms and new treatment options. However, podocytes cultured in vitro face a limited ability to maintain appreciable extents of differentiation hallmarks, raising
Alessandra Stacchiotti et al.
Nutrients, 10(6) (2018-05-31)
Taurine (TAU) is a sulfur-containing beta amino acid that is not involved in protein composition and anabolism, conditionally essential in mammals provided through diet. Growing evidence supports a protective role of TAU supply in osmoregulation, calcium flux, and reduction of
Yuqiu Lu et al.
Kidney international, 92(5), 1119-1129 (2017-07-16)
Gene expression differs substantially among individual cells of the same type. We speculate that genes that are expressed in all but a portion of cells of a given cell type would be likely essential and required for either the cell
View All Related Papers

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Product Information Sheet - P7130

Product Information Sheet

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