P8038
Proteinase, bacterial
Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
Synonym(s):
Proteinase from Bacillus licheniformis, Alkaline Protease, Protease from Bacillus licheniformis, Subtilo peptidase A
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-752-2
NACRES:
NA.54
MDL number:
Product Name
Proteinase, bacterial, Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
type
Type XXIV
form
lyophilized powder
specific activity
≤0.05 Kunitz units/mg solid RNase
≤5.0 Kunitz units/mg solid DNase
7.0-14.0 units/mg solid
mol wt
27 kDa
purified by
crystallization
shipped in
wet ice
storage temp.
−20°C
Quality Level
Related Categories
Analysis Note
Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.
Application
The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart. It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA.
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1.
Biochem/physiol Actions
Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .
General description
Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.
Other Notes
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
10 - Combustible liquids
wgk
WGK 2
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Christian Czernohlavek et al.
Biointerphases, 15(1), 011002-011002 (2020-01-18)
The implementation of self-assembled biomolecules on solid materials, in particular, sensor and electrode surfaces, gains increasing importance for the design of stable functional platforms, bioinspired materials, and biosensors. The present study reports on the formation of a planar hybrid lipid/polymer
Rebecca Anna-Maria Kenngott et al.
Cells, tissues, organs, 200(2), 153-170 (2015-05-23)
In the present investigation, bovine ovary prenatal development was studied using immunohistochemistry and laser-assisted microdissection (LAM). A major aim of this study was to evaluate the protein expression pattern of intermediate filaments (IF) and distinguish S100 protein (S100 alpha and
Silencing of cardiac mitochondrial NHE1 prevents mitochondrial permeability transition pore opening.
María C Villa-Abrille et al.
American journal of physiology. Heart and circulatory physiology, 300(4), H1237-H1251 (2011-02-08)
Inhibition of Na(+)/H(+) exchanger 1 (NHE1) reduces cardiac ischemia-reperfusion (I/R) injury and also cardiac hypertrophy and failure. Although the mechanisms underlying these NHE1-mediated effects suggest delay of mitochondrial permeability transition pore (MPTP) opening, and reduction of mitochondrial-derived superoxide production, the
Joseph W Starnes et al.
Journal of applied physiology (Bethesda, Md. : 1985), 102(5), 1793-1798 (2007-02-17)
Exercise provides cardioprotection against ischemia-reperfusion injury, a process involving mitochondrial reactive oxygen species (ROS) generation and calcium overload. This study tested the hypotheses that isolated mitochondria from hearts of endurance-trained rats have decreased ROS production and improved tolerance against Ca(2+)-induced
N V Naoumov et al.
Journal of clinical pathology, 41(7), 793-798 (1988-07-01)
A rapid and reproducible technique for in situ hybridisation, using biotin labelled probes for the Y chromosome, human DNA, hepatitis B virus DNA and cytomegalovirus DNA on formalin fixed, paraffin embedded liver tissue, was developed. The degree of proteolytic digestion
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