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Merck

P9582

Polyinosinic–polycytidylic acid potassium salt

with buffer salts, TLR ligand tested

Synonym(s):

Poly (I:C), Poly(I) • Poly(C)

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About This Item

CAS Number:
31852-29-6
UNSPSC Code:
41106305
NACRES:
NA.51
MDL number:
MFCD00131983
Assay:
≥99% (less than 1% free nucleotides, TLC)
Form:
lyophilized powder
Quality level:
300
Storage condition:
desiccated

Key Documents

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Product Name

Polyinosinic–polycytidylic acid potassium salt, with buffer salts, TLR ligand tested

assay

≥99% (less than 1% free nucleotides, TLC)

form

lyophilized powder

composition

Poly(I) • Poly(C), 10% (balance buffer salts as sodium chloride and sodium phosphate)

storage condition

desiccated

storage temp.

−20°C

Quality Level

300

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Application

TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.

Biochem/physiol Actions

Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.

Other Notes

Double-stranded homopolymer.

Packaging

Package size based on polynucleotide content

Preparation Note

The product requires ionic strength to maintain the double-strand structure. Reconstitution at ~10 mg/mL of water yields a polynucleotide in physiological phosphate buffered solution.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P9582-BULK: + P9582-50MG-PW: + P9582-50MG: + P9582-5MG-PW: + P9582-5MG: + P9582-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000486506
0000486506
0000486507
0000486507
0000426474

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Halime Kalkavan et al.
Nature communications, 8, 14447-14447 (2017-03-02)
Immune-mediated effector molecules can limit cancer growth, but lack of sustained immune activation in the tumour microenvironment restricts antitumour immunity. New therapeutic approaches that induce a strong and prolonged immune activation would represent a major immunotherapeutic advance. Here we show
William R Crum et al.
Brain, behavior, and immunity, 63, 50-59 (2016-12-13)
Genetic and environmental risk factors for psychiatric disorders are suggested to disrupt the trajectory of brain maturation during adolescence, leading to the development of psychopathology in adulthood. Rodent models are powerful tools to dissect the specific effects of such risk
Flavia S Mueller et al.
Brain, behavior, and immunity, 80, 406-418 (2019-04-14)
Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses. Evidence from investigations in
Jun-Sang Sunwoo et al.
Annals of clinical and translational neurology, 5(10), 1264-1276 (2018-10-24)
Maternal immune activation (MIA) is associated with an increased risk of autism spectrum disorder (ASD) in offspring. Herein, we investigate the altered expression of microRNAs (miRNA), and that of their target genes, in the brains of MIA mouse offspring. To
Wei-Li Wu et al.
Brain, behavior, and immunity, 62, 11-23 (2016-11-14)
Epidemiological studies show that maternal immune activation (MIA) during pregnancy is a risk factor for autism. However, mechanisms for how MIA affects brain development and behaviors in offspring remain poorly described. To determine whether placental interleukin-6 (IL-6) signaling is required
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