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PROTSIL2

ProteoSilver™ Plus Silver Stain Kit

Synonym(s):

protein stain, silver stain

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About This Item

NACRES:
NA.32
UNSPSC Code:
41105322

Key Documents

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usage

 kit sufficient for 25 mini-gels (10 × 10 cm)

Quality Level

200

technique(s)

protein staining: suitable

storage temp.

20-25°C

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General description

Conveniently packaged, extremely stable, and highly sensitive, ProteoSilver™ Plus Silver Stain Kit is an ideal product for any protein scientist. Similar to ProteoSilver (PROTSIL1), this kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver Plus leads to superior detection of low abundance proteins.
ProteoSilver Plus is also MALDI-MS compatible and does not contain glutaraldehyde, which crosslinks lysine residues, resulting in inaccurate spectra. ProteoSilver Plus Silver Stain Kit contains two additional reagents for destaining an excised protein spot, for those wishing to perform further characterization through tryptic digestion and MALDI-MS analysis.

Application

ProteoSilver™ Silver Stain Kit has been used for staining of the gels for visualizing proteins. It has also been used for destaining of the protein of interest using destainer provided in the kit for mass spectroscopic analysis.

Features and Benefits

  • Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
  • Optimized protocols help establish conditions for best results
  • High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
  • MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry
  • Room Temperature Stability allows easy and convenient storage

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Repr. 1B - Skin Corr. 1A - Skin Sens. 1 - STOT SE 2 - STOT SE 3

target_organs

Eyes,Central nervous system, Respiratory system

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

149.0 °F - closed cup

flash_point_c

65 °C - closed cup

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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pdsc

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prtr

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fsl

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ishl_indicated

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ishl_notified

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Certificates of Analysis (COA)

Lot/Batch Number
0000511621
0000511621
0000441866
0000441868
0000441868

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Slovénie Pyndiah et al.
Molecular & cellular proteomics : MCP, 6(2), 193-206 (2006-11-10)
The study of protein interactions constitutes an important domain to understand the physiology and pathogenesis of microorganisms. The two-dimensional blue native/SDS-PAGE was initially reported to analyze membrane protein complexes. In this study, both cytoplasmic and membrane complexes of a bacterium
Cédric Bernarde et al.
Molecular & cellular proteomics : MCP, 9(12), 2796-2826 (2010-07-09)
Helicobacter pylori infection plays a causal role in the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma (LG-MALT) and duodenal ulcer (DU). Although many virulence factors have been associated with DU, many questions remain unanswered regarding the evolution of the
Peeping into human renal calcium oxalate stone matrix: characterization of novel proteins involved in the intricate mechanism of urolithiasis.
Aggarwal KP, et al.
PLoS ONE, 8, e69916-e69916 (2013)
Effect of biomolecules from human renal matrix of calcium oxalate monohydrate (CaOx) stones on in vitro calcium phosphate crystallization.
Pathak P, et al.
International Brazilian Journal of Urology : Official Journal of the Brazilian Society of Urology, 36, 621-628 (2010)
Priyadarshini Pathak et al.
International braz j urol : official journal of the Brazilian Society of Urology, 36(5), 621-628 (2010-11-04)
Investigate the activity of high and low molecular weight biomolecules present in the matrix of human calcium oxalate (CaOx) stones not only on the initial mineral phase formation of calcium and phosphate (CaP) but also on its growth and demineralization
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Articles

タンパク質染色試薬選択ガイド

Sigmaでは、タンパク質可視化のためのEZBlue™ 試薬とProteoSilver™ 試薬をご提供しています。プロテオミクスや従来のPAGEフォーマットに適しています。

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

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