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Merck

R5886

RPMI-1640 Medium

HEPES modification, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217820

Key Documents

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Product Name

RPMI-1640 Medium, HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

>7.2

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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Application

RPMI-1640 Medium has been used:
  • to culture human lymphoblastoid cell line cells
  • prostate cancer cells
  • monocytes from human blood

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

R5886-1L: + R5886-PROC: + R5886PROC: + R5886-6X500ML: + R5886-PH: + R5886-500ML: + R5886-BULK: + R5886-24X500ML: + R5886-100ML: + R5886-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL9765
RNBL9605
RNBL5862
RNBL5300
RNBL3655

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