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Merck

R6750

Ribonucleic acid from baker′s yeast (S. cerevisiae)

Synonym(s):

RNA

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About This Item

CAS Number:
63231-63-0
UNSPSC Code:
41106305
NACRES:
NA.51
MDL number:
MFCD00132195
Form:
powder
Solubility:
water: 10 mg/mL, clear to very slightly hazy (faint yellow to light yellow)
Storage temp.:
−20°C

Key Documents

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Quality Level

200

form

powder

solubility

water: 10 mg/mL, clear to very slightly hazy (faint yellow to light yellow)

suitability

suitable for (suitable for use as a substrate for Rnase)

storage temp.

−20°C

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General description

Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is the substrate for RNase enzyme. The isolation of RNA from yeast is a complicated process and involves heating and freezing cycles of cells in the presence of phenyl and detergents (SDS).

Application

Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is suitable for applications such as northern blot hybridization, reverse transcriptase-polymerase chain reaction (RTPCR), and cDNA construction.

Preparation Note

Prepared by a modification of Crestfield, A.M., et al., J. Biol. Chem., 216, 185 (1955).

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

R6750-250MG: + R6750-VAR: + R6750-100MG: + R6750-BULK: + R6750-1G: + R6750-500MG-KC: + R6750-500MG:

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Certificates of Analysis (COA)

Lot/Batch Number
0000517755
0000517755
0000454152
0000454151
0000454153

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Kazuya Shirato et al.
Virology journal, 11, 139-139 (2014-08-12)
The first documented case of Middle East Respiratory Syndrome coronavirus (MERS-CoV) occurred in 2012, and outbreaks have continued ever since, mainly in Saudi Arabia. MERS-CoV is primarily diagnosed using a real-time RT-PCR assay, with at least two different genomic targets
Radha Duttagupta et al.
PloS one, 7(2), e31241-e31241 (2012-02-24)
Inflammatory Bowel Disease--comprised of Crohn's Disease and Ulcerative Colitis (UC)--is a complex, multi-factorial inflammatory disorder of the gastrointestinal tract. In this study we have explored the utility of naturally occurring circulating miRNAs as potential blood-based biomarkers for non-invasive prediction of
Michael R Green et al.
Cold Spring Harbor protocols, 2021(12) (2021-12-03)
Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
Stephanie C Bannister et al.
Biology of reproduction, 81(1), 165-176 (2009-04-10)
MicroRNAs are a highly conserved class of small RNAs that function in a sequence-specific manner to posttranscriptionally regulate gene expression. Tissue-specific miRNA expression studies have discovered numerous functions for miRNAs in various aspects of embryogenesis, but a role for miRNAs
Douglas A Weigent
Cellular immunology, 282(2), 71-78 (2013-06-19)
In the present study, we show that higher levels of lymphocyte GH are expressed in spleen cells from aging animals compared to young animals. Further, leukocytes from primary and secondary immune tissues and splenic T and B cells from aging
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Protocols

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

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