Skip to Content
Merck

R7388

RPMI-1640 Medium

With HEPES, with ʟ-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217820

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

RPMI-1640 Medium, Modified, with 20 mM HEPES and L-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

>7.2

components

HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
RPMI-1640 medium formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. It is used for the culture of human normal and neoplastic leukocytes. RPMI-1640 also supports the growth of various types of cell cultures, like fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

Application

RPMI-1640 medium has been used to culture:
  • human immortalized keratinocyte cells and in in vitro transfection assay
  • cell lines and human T-cell blasts and to monitor autophagic flux
  • peripheral blood mononuclear cells of piglets (PBMCs)

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

R7388-PROC: + R7388-1L: + R7388-100ML: + R7388-BULK: + R7388-500ML: + R7388-VAR: + R7388-6X500ML:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
RNBM1127
RNBL9579
RNBL9043
RNBL5863
RNBL3005

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Patrick Linder et al.
PLoS genetics, 10(2), e1004207-e1004207 (2014-03-04)
RNA decay and maturation have in recent years been recognised as major regulatory mechanisms in bacteria. In contrast to Escherichia coli, the Firmicute (Gram-positive) bacteria often do not encode the well-studied endonuclease RNase E, but instead rely on the endonucleases
Janina Treffon et al.
Cellular microbiology, 22(5), e13158-e13158 (2020-01-03)
Staphylococcus aureus is one of the earliest pathogens that persists the airways of cystic fibrosis (CF) patients and contributes to increased inflammation and decreased lung function. In contrast to other staphylococci, S. aureus possesses two superoxide dismutases (SODs), SodA and
Christine Silvia Gibhardt et al.
Cell reports, 33(3), 108292-108292 (2020-10-22)
Store-operated calcium entry (SOCE) through STIM-gated ORAI channels governs vital cellular functions. In this context, SOCE controls cellular redox signaling and is itself regulated by redox modifications. However, the molecular mechanisms underlying this calcium-redox interplay and the functional outcomes are
Maarit Suomalainen et al.
Journal of cell science, 134(5) (2020-09-13)
In clonal cultures, not all cells are equally susceptible to virus infection, and the mechanisms underlying this are poorly understood. Here, we developed image-based single-cell measurements to scrutinize the heterogeneity of adenovirus (AdV) infection. AdV delivers, transcribes and replicates a
Heizel Rosado-Galindo et al.
Recent progress in materials, 2(2) (2020-01-01)
Cells can respond to different topographical cues in their natural microenvironment. Hence, scientists have employed microfabrication techniques and materials to generate culture substrates containing topographies for cell-based assays. However, one of the limitations of custom topographical platforms is the lack
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service