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Merck

S0677

Streptavidin from Streptomyces avidinii

recombinant, expressed in E. coli, lyophilized powder

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About This Item

CAS Number:
9013-20-1
UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00082035
Form:
lyophilized powder
Recombinant:
expressed in E. coli

Key Documents

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Quality Level

200

recombinant

expressed in E. coli

form

lyophilized powder

capacity

4 mol/mol binding capacity (Biotin)

storage temp.

−20°C

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General description

Streptavidin is a biotin-binding protein that is obtained from Streptomyces avidinii. It is resistant to extreme temperature, pH, detergents and proteolytic enzymes. Streptavidin has a molecular weight of 60kDa and exists as a homotetramer.

Application

Streptavidin from Streptomyces avidinii has been used-
  • for blocking of the AFM (atomic force microscopy) tip, during the measurement of unbinding forces in the avidin-biotin system
  • for coupling of anti-HA (hemagglutinin) antibodies during cell free synthesis and assembly of proteins on a biochip
  • for the coating of the gelatin flow phantoms to eventually determine the feasibility of IVUS (intravascular ultrasound) transducer for (biotinylated) microbubble-based drug delivery
  • to immobilize proteins and oligonucleotides

Biochem/physiol Actions

Streptavidin is an antibiotic that functions by binding to and depleting the essential vitamin biotin from the surrounding environment. Because of its unique properties, streptavidin has found various applications in biological studies, including immunotherapy, immunoassays, hybridization assays, lymphocyte activation, antigen localization and affinity chromatography.

Physical form

Lyophilized from 5 mM potassium phosphate buffer

Other Notes

One unit will bind 1.0 μg biotin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

S0677-VAR: + S0677-25MG: + S0677-BULK: + S0677-1MG: + S0677-5MG:

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Certificates of Analysis (COA)

Lot/Batch Number
0000491226
0000491223
0000491224
0000491224
0000491226

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Nicola Ilk et al.
Applied and environmental microbiology, 68(7), 3251-3260 (2002-06-29)
The nucleotide sequence encoding the crystalline bacterial cell surface (S-layer) protein SbpA of Bacillus sphaericus CCM 2177 was determined by a PCR-based technique using four overlapping fragments. The entire sbpA sequence indicated one open reading frame of 3,804 bp encoding
Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide Ligation
Nuttada Panpradist
PLoS ONE (2016)
Identification of the Heparan Sulfate Binding Sites in the Cellular Prion Protein*
Richard G
The Journal of Biological Chemistry (2002)
Determination of binding curves via protein micropatterning in vitro and in living cells
Stefan S
Cytometry (2012)
The properties of streptavidin, a biotin-binding protein produced by Streptomycetes
Louis C
Archives of Biochemistry and Biophysics, 106 (1964)
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