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Merck

S8398

Shields and Sang M3 Insect Medium

With L-glutamine, without potassium bicarbonate, powder, suitable for insect cell culture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00243218

Key Documents

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form

powder

technique(s)

cell culture | insect: suitable

components

NaHCO3: no
L-glutamine: 0.6 g/L
glucose: 10 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

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General description

Shields and Sang M3 insect medium can support the rapid growth of both primary and established cultures of Drosophila melanogaster cells. Insect tissue culture media have been formulated to mimic the main properties of the hemolymph of specific insects.

Application

Developed for the culture of Drosophila cell lines.
Shields and Sang M3 Insect Medium has been used:
  • for the dissection of Drosophila larvae to study the changes in the translational activity of larval wing imaginal disc cells
  • to incubate Drosophila bodies for surface sensing of translation (SUnSET) assay
  • to culture Drosophila cells for transfection studies

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

S8398-25K: + S8398-VAR: + S8398-100L: + S8398-BULK: + S8398-1L-KC: + S8398-50K: + S8398-50L: + S8398-2X5L: + S8398-PROC: + S8398-5L: + S8398-BIBC: + S8398LF1-BIBC: + S8398-10L: + S8398-25KG: + S8398-10X1L: + S8398-50KG: + S8398-1L:

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Certificates of Analysis (COA)

Lot/Batch Number
0000513586
0000513586
0000513584
0000513584
0000487868

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Louis Philip Benoit Bouvrette et al.
RNA (New York, N.Y.), 24(1), 98-113 (2017-10-29)
Cells are highly asymmetrical, a feature that relies on the sorting of molecular constituents, including proteins, lipids, and nucleic acids, to distinct subcellular locales. The localization of RNA molecules is an important layer of gene regulation required to modulate localized
Tim Hundertmark et al.
PloS one, 13(9), e0203622-e0203622 (2018-09-08)
Spermatogenesis in many species including Drosophila melanogaster is accompanied by major reorganisation of chromatin in post-meiotic stages, involving a nearly genome-wide displacement of histones by protamines, Mst77F and Protamine-like 99C. A proposed prerequisite for the histone-to-protamine transition is massive histone
Ahmed Sabri et al.
PloS one, 6(4), e18847-e18847 (2011-04-29)
Aphidius ervi (Hymenoptera: Braconidae) is an entomophagous parasitoid known to be an effective parasitoid of several aphid species of economic importance. A reduction of its production cost during mass rearing for inundative release is needed to improve its use in
Stefanie M K Gärtner et al.
European journal of cell biology, 94(1), 46-59 (2014-12-04)
Spermatogenesis is accompanied by a remarkable reorganization of the chromatin in post-meiotic stages, characterized by a near genome-wide displacement of histones by protamines and a transient expression of transition proteins. In Drosophila, the transition-protein-like protein Tpl94D contains an HMG-box domain
Maria-Del-Carmen Diaz-de-la-Loza et al.
Development (Cambridge, England), 147(5) (2020-03-04)
Mutations in the Ultrabithorax (Ubx) gene cause homeotic transformation of the normally two-winged Drosophila into a four-winged mutant fly. Ubx encodes a HOX family transcription factor that specifies segment identity, including transformation of the second set of wings into rudimentary
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