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Merck

S9751

Sulfatase from Helix pomatia

Type H-2, aqueous solution, ≥2,000 units/mL

Synonym(s):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

CAS Number:
9016-17-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.1.6.1 (BRENDA, IUBMB)
MDL number:
MFCD00132399
Concentration:
≥2,000 units/mL

Key Documents

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type

Type H-2

form

aqueous solution

secondary activity

≥100,000 units/mL β-glucuronidase (at pH 5.0)

mol wt

~85 kDa by gel filtration

concentration

≥2,000 units/mL

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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General description

Sequential action of sulfatase from Helix pomatia and β-O-glucosidase from Caldicellulosiruptor saccharolyticus on glucosinolates allows synthesis of thiohydroximates. Sulfatase from Helix pomatia hydrolyzes β-naphthyl sulfate much faster than α-naphthyl sulfate.

Application

Sulfatase from Helix pomatia is often used to remove sulfate groups from carbohydrates.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

S9751-5ML: + S9751-VAR: + S9751-10ML: + S9751-BULK: + S9751-25ML: + S9751-1ML: + S9751-2ML:

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Certificates of Analysis (COA)

Lot/Batch Number
0000536398
0000536397
0000521077
0000521077
0000536398

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Fernando Cámara-Martos et al.
Foods (Basel, Switzerland), 11(3) (2022-02-16)
In northwest Spain and Portugal, there is a long tradition of cultivating B. rapa subsp. rapa to obtain turnip greens and turnip tops. Brassica rapa L. subsp. rapa (turnip greens and turnip tops) were grown under conventional and organic conditions
Tania Merinas-Amo et al.
Foods (Basel, Switzerland), 10(11) (2021-11-28)
Brassica rapa L. subsp. rapa (turnip greens), a traditionally consumed vegetable, is well-known due to its high content of glucosinolates, which are secondary metabolites with a positive biological activity for human health. Our hypothesis has been based on the relation
Abigail M Tokheim et al.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 18(5), 537-540 (2005-12-08)
Often used to remove sulfate groups from carbohydrates, the regulatory properties of the aryl sulfatase from Helix pomatia remain little characterized. As many hydrolytic enzymes utilize exogenous metal ions in catalysis, the effect of various divalent metal ions on the
Jakub Kopycki et al.
Biotechnology letters, 33(5), 1039-1046 (2011-01-27)
Thiohydroximates comprise a diverse class of compounds important in both biological and industrial chemistry. Their syntheses are generally limited to simple alkyl and aryl compounds with few stereocenters and a narrow range of functional groups. We hypothesized that sequential action
Monica L Vermillion Maier et al.
Drug metabolism and disposition: the biological fate of chemicals, 49(8), 694-705 (2021-05-27)
3,3'-Diindolylmethane (DIM), a major phytochemical derived from ingestion of cruciferous vegetables, is also a dietary supplement. In preclinical models, DIM is an effective cancer chemopreventive agent and has been studied in a number of clinical trials. Previous pharmacokinetic studies in
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Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

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